GC–MS Analysis and In Vitro Evaluation of Antioxidant and Cytotoxic Activities of Melaleuca viminalis (Myrtaceae)

• Published in: Journal of plant biochemistry and biotechnology Vol. 31; no. 2; pp. 453 - 458
• Main Authors: Darwish, Ahmed G., Hassan, Hanaa M., Samy, Mamdouh Nabil, Shaker, Emad S., Basha, Sheikh Mehboob, Balasubramani, Subramani Paranthaman
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01-06-2022; Springer Nature B.V
• Subjects: Acetic acid; Anticancer properties; Antioxidants; Antitumor activity; Biological activity; Biomedical and Life Sciences; Butanol; Cell Biology; Chemical fractionation; Chloroform; Cytotoxicity; Ethyl acetate; Flavonoids; Fractionation; Gas chromatography; Hexanes; Life Sciences; Mass spectrometry; Mass spectroscopy; Melaleuca; n-Hexane; Phenolic compounds; Phenols; Plant Biochemistry; Protein Science; Receptors; Scavenging; Short Communication; Vitamin E; HepG2; GC–MS; FRAP; DPPH; A549
• ISSN: 0971-7811; 0974-1275
• DOI: 10.1007/s13562-021-00700-1

Melaleuca viminalis
is a medicinal shrub used in traditional medicine globally for the treatment of numerous diseases. Gas chromatography–mass spectrometry results revealed the presence of 23 phytoconstituents in methanolic extract. The chemical fractionation of the methanolic extract (ME) using
n-
hexane, chloroform, ethyl acetate,
n-
butanol, and water was prepared and analyzed for phytochemical content and bioactivity. Ethyl acetate fraction was found to contain the highest phenolic and flavonoid contents (4.81 ± 0.01 mg GAE/ and 3.32 ± 0.03 mg QUR/, respectively), in vitro antioxidant radical scavenging activity DPPH with value 97.1 ± 0.61%, FRAP activity 4048 ± 0.13 μM Trolox/g, comparable with the standard Trolox, and showed the highest anticancer activity against two different human cancer cells A549 and HepG2 (97 ± 5.3 and 98 ± 3.8%, respectively). Results of this study displayed that ethyl acetate (EA) fraction of
M. viminalis
are the rich source of phenolics and other compounds that can play a significant role in preventing the progression of many diseases.