Synthesis, characterization and protective effect of green tea-mediated zinc oxide nanoparticles against ochratoxin A induced hepatotoxicity and nephrotoxicity in albino rats

• Published in: Applied nanoscience Vol. 11; no. 8; pp. 2281 - 2289
• Main Authors: Hassan, Syeda Aamna, Mujahid, Huma, Ali, Muhammad Muddassir, Irshad, Shagufta, Naseer, Rahat, Saeed, Shagufta, Firyal, Sehrish, Arooj, Fariha
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-08-2021; Springer Nature B.V
• Subjects: Albumins; Chemistry and Materials Science; Conversion ratio; Creatinine; Degeneration; Fermentation; Fourier transforms; Functional groups; Green tea; Infrared spectroscopy; Kidneys; Liver; Lymphocytes; Materials Science; Membrane Biology; Nanochemistry; Nanoparticles; Nanotechnology; Nanotechnology and Microengineering; Necrosis; Original Article; Scanning electron microscopy; Spectrum analysis; Synthesis; Tea; X-ray diffraction; Zinc oxide; Zinc oxides; Albino rats; Ochratoxin A; Zinc oxide nanoparticles
• ISSN: 2190-5509; 2190-5517
• DOI: 10.1007/s13204-021-02006-z

The present study describes the synthesis, characterization and antiochratoxin efficacy of zinc oxide nanoparticles against hepatotoxicity and nephrotoxicity in albino rats. Ochratoxin A (OTA) was produced by solid state fermentation on wheat grains using
Aspergillus ochraceus
and estimated by HPLC. ZnONPs were produced using leaf extract of
Camellia sinensis
and characterized by UV–visible spectrophotometer, Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and X-ray diffraction (XRD). Different doses were given to albino rats by dividing them into different groups i.e., group A (basal feed), group B (50 ppb OTA), group C (50 ppb OTA and 25 ppb ZnONPs) and group D (25 ppb ZnONPs). Blood was collected for biochemical analysis and tissues of liver and kidney were taken for histopathological examination. UV–visible spectroscopy showed prominent peak of green synthesized nanoparticles having maximum absorption of 350 nm and size range from 30 to 40 nm using SEM. The synthesized ZnO nanostructure was confirmed by XRD image and different functional groups responsible for green synthesis were confirmed by FTIR analysis. OTA significantly decreased the weight gain, feed consumption and feed conversion ratio of rats. Histopathological examination of liver and kidney indicated that OTA has severe effects including degeneration, increase sinusoidal spaces, infiltration of lymphocytes, vascular congestion and necrosis. Biochemical parameters such as ALT, AST and creatinine were significantly raised whereas albumin and total protein were decreased by OTA feeding. These changes were ameliorated by green synthesized ZnONPs. This study concluded that OTA severely affect liver and kidney of rats and green tea mediated ZnONPs plays a hepatoprotective and nephroprotective role to reduce the lethal effects of OTA in albino rats.