Effect of microalga Desmodesmus subspicatus and plant growth regulators on the in vitro propagation of Cattleya warneri

• Published in: Plant cell, tissue and organ culture Vol. 153; no. 1; pp. 77 - 89
• Main Authors: Navarro, Quezia Rocha, de Oliveira Corrêa, Diego, Behling, Alexandre, Noseda, Miguel Daniel, Ribas, Luciana Lopes Fortes
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-04-2023; Springer Nature B.V
• Subjects: Acclimatization; Algae; Aquatic microorganisms; Benzyladenine; Biomass; Biomedical and Life Sciences; Cattleya; Crataegus warneri; Desmodesmus subspicatus; Explants; Growth regulators; Life Sciences; Microalgae; Micropropagation; Naphthaleneacetic acid; Original Article; Plant Genetics and Genomics; Plant growth; Plant Pathology; Plant Physiology; Plant propagation; Plant Sciences; Propagation; Protocorm-like bodies; Regeneration; Seedlings; Substrates; Survival; Threatened species; Vermiculite; Microalgal biomass; Micropropagation; Orchidaceae; Protocorm; Microalgal extract
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-022-02442-x

Cattleya warneri
, an orchid with ornamental potential, suffers indiscriminate harvest and is classified as a vulnerable species, requiring propagation studies. The aim of this study was to evaluate the effect of biomass or aqueous extract of
Desmodesmus subspicatus
microalga and plant growth regulators (PGRs): 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA) on the in vitro propagation of
C. warneri
using protocorms as explants. Entire protocorms and sectioned transversely into two halves: apical and basal or longitudinal halves were grown on MS culture medium, with the concentration of macronutrients reduced by half (MSM/2) containing
D. subspicatus
biomass or extract and BA and/or NAA. The results indicated that the apical halves of protocorm were the best explants and the entire protocorms formed seedlings. The regeneration of protocorm-like bodies (PLBs) was more efficient with the addition of 1.5 g L
−1
of microalgae biomass (92% and 4.7 PLBs) or 1.0 g L
−1
of extract (100% and 3.3 PLBs) and 8 µM BA (89% and 5.2 PLBs). The biomass (1.0 g L
−1
) or extract (2.0 g L
−1
) also enabled PLBs regeneration from entire protocorms (70% and 4.0 PLBs, 50% and 3.2 PLBs, respectively). The plant acclimatization was recommended using coconut fiber and vermiculite (1:1, v/v) as substrate (survival rate 84% and greater fresh mass 0.895 g). In conclusion, a rapid and efficient protocol for the mass propagation of
C. warn
eri was achieved using apical halves of protocorms, and MSM/2 medium supplementation with biomass or extract of
D. subspicatus
is an effective alternative to replace conventional PGRs.
Key message
Complete protocol for micropropagation of
Cattleya warneri
using PLBs regeneration was established. Apical halves of protocorms were the most responsive explant and
Desmodesmus subspicatus
microalga is alternative to replace PGRs.