A cell-based protein-protein interaction method using a permuted luciferase reporter

We have developed a novel cell-based protein-protein interaction assay method. The method relies on conversion of an inactive permuted luciferase containing a Tobacco Etch Virus protease (TEV) cleavage sequence fused onto protein (A) to an active luciferase upon interaction and cleavage by another p...

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Bibliographic Details
Published in:	Current chemical genomics Vol. 5; pp. 122 - 128
Main Authors:	Eishingdrelo, Haifeng, Cai, Jidong, Weissensee, Paul, Sharma, Praveen, Tocci, Michael J, Wright, Paul S
Format:	Journal Article
Language:	English
Published:	United Arab Emirates Bentham Open 2011
Subjects:	permuted luciferase cell signaling RTK GPCR NHR Cell-based assay beta-arrestins protein-protein interactions
Online Access:	Get full text
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Summary:	We have developed a novel cell-based protein-protein interaction assay method. The method relies on conversion of an inactive permuted luciferase containing a Tobacco Etch Virus protease (TEV) cleavage sequence fused onto protein (A) to an active luciferase upon interaction and cleavage by another protein (B) fused with the TEV protease. We demonstrate assay applicability for ligand-induced protein-protein interactions including G-protein coupled receptors, receptor tyrosine kinases and nuclear hormone receptors.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1875-3973 1875-3973
DOI:	10.2174/1875397301105010122