Synthesis and characterization of glycoconjugate tin(IV) complexes: In vitro DNA binding studies, cytotoxicity, and cell death

• Published in: Journal of organometallic chemistry Vol. 696; no. 8; pp. 1600 - 1608
• Main Authors: Tabassum, Sartaj, Khan, Rais Ahmad, Arjmand, Farukh, Sen, Subrata, Kayal, Jyoti, Juvekar, Aarti S., Zingde, Surekha M.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15-04-2011
• Subjects: Cytotoxicity; Glycoconjugate–Sn(IV) complexes; In vitro DNA binding studies; Nuclear morphology; Cytotoxicity; Nuclear morphology; Glycoconjugate–Sn(IV) complexes; In vitro DNA binding studies
• ISSN: 0022-328X; 1872-8561
• DOI: 10.1016/j.jorganchem.2011.01.012

New glycosyl derived ligand and its complexes, with SnCl
4·5H
2O
(1) and (CH
3)
2SnCl
2
(2) were synthesized and characterized by spectroscopic (IR,
1H,
13C, and
119Sn NMR, UV–vis, ESI-MS) and analytical methods. Interaction studies of
1 and
2 with CT DNA were studied by using various biophysical techniques, which showed high binding affinity of
2 with CT DNA.
In vitro cytotoxicity of complexes
1 and
2 were evaluated against different human cancer cell lines of different histological origins by employing SRB Assay. The organotin(IV) complex
2 exhibited remarkable activity against DWD (oral cancer) cell lines with GI
50 values <10
μg/ml. Complex
2 induced apoptosis of DWD cell line at a very low concentration of 1–4
μg/mL.
New glycosyl derived organotin antitumor drug candidate
2 has been designed and synthesized, which possesses remarkable
in vitro antitumor activity and induces apoptosis of DWD cell line at a very low concentration of 1–4
μg/mL.
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► New glycosyl derived ligand and its complexes, with SnCl
4·5H
2O
(1) and (CH
3)
2SnCl
2
(2) were synthesized and characterized by spectroscopic (IR,
1H,
13C, and
119Sn NMR, UV–vis, ESI-MS) and analytical methods. ► Interaction studies of
1 and
2 with CT DNA were studied by using various biophysical techniques, which showed high binding affinity of
2 with CT DNA. ►
In vitro cytotoxicity of complexes
1 and
2 were evaluated against different human cancer cell lines of different histological origins by employing SRB Assay. ► The organotin(IV) complex
2 exhibited remarkable activity against DWD (oral cancer) cell lines with GI
50 values <10
μg/ml. ► Complex
2 induced apoptosis of DWD cell line at a very low concentration of 1–4
μg/mL.