Chromosomal assignment of the human genes coding for the major proteins of the desmosome junction, desmoglein DGI (DSG), desmocollins DGII/III (DSC), desmoplakins DPI/II (DSP), and plakoglobin DPIII (JUP)

• Published in: Genomics (San Diego, Calif.) Vol. 10; no. 3; p. 640
• Main Authors: Arnemann, J, Spurr, N K, Wheeler, G N, Parker, A E, Buxton, R S
• Format: Journal Article
• Language: English
• Published: United States 01-07-1991
• Subjects: Animals; Base Sequence; Chromosome Mapping; Chromosomes, Human, Pair 18; Chromosomes, Human, Pair 6; Chromosomes, Human, Pair 7; Chromosomes, Human, Pair 9; Cytoskeletal Proteins - genetics; Desmocollins; Desmogleins; Desmoplakins; Desmosomes; gamma Catenin; Genes; Humans; Hybrid Cells; Mice; Molecular Sequence Data; Polymerase Chain Reaction; Rats
• ISSN: 0888-7543
• DOI: 10.1016/0888-7543(91)90446-L

We have established PCR assays for the genes coding for the major proteins of the desmosome type of cell junction, the desmosomal cadherins DGI (desmoglein) and DGII/III (desmocollins), and the plaque proteins DPI/II (desmoplakin) and DPIII (plakoglobin) and used them to test human-mouse and human-rat somatic cell hybrids with different contents of human chromosomes. From these data we were able to assign DGI to chromosome 18 (DSG), DGII/III to chromosome 9p (DSC), DPI/II to chromosome 6p21-ter(DSP), and DPIII to chromosome 7 (JUP).