A phytochemical study of lignans in whole plants and cell suspension cultures of Anthriscus sylvestris

• Published in: Planta medica Vol. 69; no. 8; p. 733
• Main Authors: Koulman, Albert, Kubbinga, Marlies E, Batterman, Sieb, Woerdenbag, Herman J, Pras, Niesko, Woolley, Jack G, Quax, Wim J
• Format: Journal Article
• Language: English
• Published: Germany 01-08-2003
• Subjects: Apiaceae - cytology; Apiaceae - metabolism; Cell Culture Techniques; Cell Line; Cells, Cultured; Chromatography, High Pressure Liquid; Gas Chromatography-Mass Spectrometry; Humans; Lignans - analysis; Lignans - biosynthesis; Phytotherapy; Plant Structures - cytology; Plant Structures - metabolism
• ISSN: 0032-0943
• DOI: 10.1055/s-2003-42776

In the roots of Anthriscus sylvestris 12 different lignans were detected. Arctigenin, dimethylmatairesinol, dimethylthujaplicatin, podophyllotoxin, 7-hydroxyyatein and 7-hydroxyanhydropodorhizol have not been previously reported to be present in A. sylvestris. In the cell suspension cultures, which were initiated for this study, trace amounts of deoxypodophyllotoxin could be detected. With these cell suspension cultures we carried out feeding experiments using deoxypodophyllotoxin, yatein and, anhydropodorhizol. Yatein had a toxic effect on the cell cultures and was, like anhydropodorhizol, not converted into any detectable product. Deoxypodophyllotoxin, in contrast, was converted into podophyllotoxin, yielding significantly higher concentration than measured in whole plants.