Coat protein-independent cell-to-cell movement of bromoviruses expressing brome mosaic virus movement protein with an adaptation-related amino acid change in the central region

• Published in: Archives of virology Vol. 150; no. 6; pp. 1231 - 1240
• Main Authors: SASAKI, N, KAIDO, M, OKUNO, T, MISE, K
• Format: Journal Article
• Language: English
• Published: Wien Springer 01-06-2005; New York, NY Springer Nature B.V
• Subjects: Adaptation; Adaptation, Physiological; Amino Acid Sequence; Amino acids; Biological and medical sciences; Brome mosaic virus; Bromovirus - genetics; Bromovirus - metabolism; Bromovirus - pathogenicity; Bromovirus - physiology; Capsid Proteins - metabolism; Chenopodium quinoa - virology; Codon; Cowpea chlorotic mottle virus; Flowers & plants; Fundamental and applied biological sciences. Psychology; Genes; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Infections; Microbiology; Miscellaneous; Mutation; Plant Viral Movement Proteins; Plasmids; Proteins; Recombination, Genetic; Viral infections; Viral Proteins - chemistry; Viral Proteins - genetics; Viral Proteins - metabolism; Virology; Viruses; Bromovirus; Virus; Plant pathogen; Brome mosaic virus; Cowpea chlorotic mottle virus; Bromoviridae; Property structure relationship; Capsid; Protein; Adaptation
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-004-0481-x

The movement protein (MP) of Brome mosaic virus (BMV) depends on the coat protein (CP) to mediate the cell-to-cell movement of BMV and CCMV(B3a), a recombinant Cowpea chlorotic mottle virus (CCMV) expressing BMV MP. Previous studies identified gain-of-function mutations in the central region of BMV MP that enable CCMV(B3a) to adapt to a resistant host. This study demonstrates that all adaptation-related MPs can partially or almost fully mediate the cell-to-cell movement of CCMV(B3a) and BMV without CP. Based on these results, we discuss adaptation mechanisms of CCMV(B3a) and the role of the central region of MP in the determination of virus movement mode.