Different compact hybrid Langmuir-Blodgett-film coatings modify biomineralization and the ability of osteoblasts to grow

• Published in: Journal of biomedical materials research. Part B, Applied biomaterials Vol. 106; no. 7; pp. 2524 - 2534
• Main Authors: de Faria, Amanda N, Cruz, Marcos A E, Ruiz, Gília C M, Zancanela, Daniela C, Ciancaglini, Pietro, Ramos, Ana P
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01-10-2018
• Subjects: Biocompatibility; Biomaterials; Biomedical materials; Biomimetics; Calcium; Calcium ions; Calcium phosphates; Cell culture; Cell differentiation; Chemical composition; Chemical properties; Coatings; Differentiation (biology); Hydroxyapatite; Langmuir-Blodgett films; Materials research; Materials science; Mineralization; Organic chemistry; Osteoblastogenesis; Osteoblasts; Phosphates; Phospholipids; Regeneration; Tissue engineering; hydroxyapatite; osteoblasts; biomineralization; titanium; Langmuir-Blodgett
• ISSN: 1552-4973; 1552-4981
• DOI: 10.1002/jbm.b.34069

Calcium phosphates (CaPs) are biomaterials widely used in tissue regeneration with outstanding biological performance. Although the tremendous improvements achieved in CaP's materials research over the years, their interaction with physiological environments still need to be fully understood. The aim of this study is to explore a biomimetic Langmuir-Blodgett (LB) membrane to template the growth of hydroxyapatite (HAp) coatings on Ti surfaces and the ability of these coatings in inducing biomineralization by osteoblasts cultured in vitro. Changing the phospholipids (i.e., dihexadecyl phosphate (DHP) or octadecylphosphonic acid (OPA)), we also tuned the surface Ca
concentration. This structural feature gave rise to different LB-hybrid surfaces where the concentration of Ca
in the OPA/HAp was higher than the concentration of Ca
in DHP/HAp coating. The higher Ca
amount on OPA/HAp coatings, allied to the physical-chemical features, lead to different responses on osteoblasts, stimulating or inhibiting the natural biomineralization. The OPA/HAp coating caused a delay in the osteoblast proliferation as indicated by the decrease in the cell viability at the 7th culture day. Improved cell differentiation triggered by the DHP/HAp coating resulted in higher osteoblast biomineralization. The present data underscore that besides both coatings being composed by HAp, the final interfacial composition and physical-chemical properties influence differently the osteoblast behavior. Although the best osteoblast's viability was found to OPA/HAp, our dataset attested that DHP/HAp induced mineralization more effectively than that. This unexpected finding highlight the importance of deeply understanding the biomaterial interface and suggest a promising approach to the design of biofunctional LB-based coatings with tunable properties. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2524-2534, 2018.