Pathogenicity of indigenous Beauveria bassiana (Balsamo) against Ceratitis capitata Wiedemann (Diptera: Tephritidae) under laboratory conditions

• Published in: Egyptian journal of biological pest control Vol. 30; no. 1; pp. 1 - 7
• Main Authors: Chergui, Sabrina, Boudjemaa, Khadidja, Benzehra, Abdelmadjid, Karaca, Ismail
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 14-10-2020; SpringerOpen
• Subjects: Bioassay; Biomedical and Life Sciences; Ceratitis capitata; Entomopathogenic fungi; Life Sciences; Native isolate; Pathogenicity; Zoology; Pathogenicity; Bioassay; Entomopathogenic fungi; Native isolate
• ISSN: 2536-9342; 2536-9342
• DOI: 10.1186/s41938-020-00331-z

Background
The Mediterranean fruit fly,
Ceratitis capitata
(Wiedemann) (Diptera: Tephritidae), is the major insect pest of fruit production worldwide. Chemical control of this pest has induced the selection of resistant medflies populations and negative environmental impact. In addition, the use of pesticides has become an obstacle to the fresh agricultural products exported to many countries that restrict pesticides residues. The aim of this study was to propose alternatives based on the use of microbiological products for the control of
C. capitata
.
Main body
The pathogenicity of the Turkish isolate
Beauveria bassiana
BMAUM M6-4 was evaluated against immature stages and adult of
C. capitata
under laboratory conditions at a concentration of 10
7
conidia/ml via different routes of exposure. Conidial treatment was applied by spraying soil and filter paper against 3rd instar larvae and pupae. In contrast, the treatment was administered to adult males and females by contact and ingestion. The percentage of mortality was recorded on the 3rd, 7th, 12th and 15th day after inoculation
.
Obtained results showed that
B. bassiana
was very effective against the adult’s fly, where males were more susceptible than females in both treatments (contact and ingestion). This isolate was tested against 3rd instar larvae. It achieved an infection rate of 33.33 and 43.5% of the larvae treated on filter paper and in the soil, respectively. Application of conidial preparations to soil reduced insect emergence and CAN represent a promising strategy for fruit fly integrated management (57.33 and 56.66% emergence from the treatments in soil and filter paper).
Conclusion
Entomopathogenic fungi are promising as a biocontrol agent that can be used under different modes of ground application against larvae, prepupae and pupae, and/or as a bait spraying or contact against adults.