The Neuroprotective Effect of N-Docosahexaenoyldopamine on Degenerating Dopaminergic Neurons of the Mesencephalon

• Published in: Biology bulletin of the Russian Academy of Sciences Vol. 47; no. 5; pp. 466 - 473
• Main Authors: Surkov, S. A., Mingazov, E. R., Blokhin, V. E., Sturova, A. I., Gretskaya, N. M., Zinchenko, G. N., Bezuglov, V. V., Ugrumov, M. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-09-2020; Springer Nature B.V
• Subjects: Axons; Biochemistry; Biomedical and Life Sciences; Cell Biology; Cell culture; Dopamine; Dopamine receptors; Ecology; Fetuses; Life Sciences; Mesencephalon; MPP; Neuritis; Neurons; Neuroprotection; Sodium chloride; Zoology
• ISSN: 1062-3590; 1608-3059
• DOI: 10.1134/S1062359020050106

This study was aimed at evaluating the neuroprotective effect of
N
-docosahexaenoyldopamine (DHA-DA), an endogenous bioactive lipid of the neurolipin family, on mesencephalic dopaminergic neurons of mouse fetuses in a cell culture under exposure to 1-methyl-4-phenylpyridinium
+
(MPP
+
), a specific neurotoxin for catecholaminergic neurons. The state of neurons in the experiment (MPP
+
) and in the control (0.9% NaCl) was estimated according to three parameters: the number of surviving dopaminergic neurons, the length of the neurites, and the total content of dopamine in the neurons. DHA-DA was shown to have a dose-dependent neuroprotective effect on degenerating dopaminergic neurons under the influence of MPP
+
. DHA-DA at concentrations of 0.5 μM or lower does not have a neuroprotective effect. However, at higher concentrations it has a neuroprotective effect, preventing the death of dopaminergic neurons and the degradation of neuritis. At a concentration of 1 μM, DHA-DA protects the neuron cell bodies from degradation to a higher extent than their processes, whereas at a concentration 2 μM it protects and/or stimulates to a greater extent the growth of processes of the surviving neurons. In addition, DHA-DA reduces significantly the loss of dopamine in neurons under exposure to MPP
+
. Thus, it is shown that DHA-DA has a dose-dependent neuroprotective effect on degenerating dopaminergic neurons.