Characteristics of receptor- and transducer-coupled activation of the intracellular signalling in sensory neuron revealed by atomic force microscopy

• Published in: Technical physics letters Vol. 43; no. 1; pp. 85 - 87
• Main Authors: Khalisov, M. M., Penniyaynen, V. A., Esikova, N. A., Ankudinov, A. V., Krylov, B. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 2017; Springer Nature B.V
• Subjects: Atomic force microscopy; Classical and Continuum Physics; Mechanical properties; Physics; Physics and Astronomy; Receptors; Signal transmission; Transducers
• ISSN: 1063-7850; 1090-6533
• DOI: 10.1134/S1063785017010072

The mechanical properties of sensory neurons upon activation of intracellular cascade processes by comenic acid binding to a membrane opioid-like receptor (receptor-coupled), as well as a very low (endogenous) concentration of ouabain (transducer-coupled), have been investigated. Using atomic force microscopy, it is established that exposure to ouabain, in contrast to the impact of comenic acid, leads to a hardening of the neuron soma. This suggests that the receptor-coupled signal transmission to the cell genome is carried out through mechanisms that are different from the transducer-coupled signal pathways.