Prospects for the use of new basidiomycete strains for the direct conversion of lignocellulose into ethanol

• Published in: Applied biochemistry and microbiology Vol. 53; no. 5; pp. 557 - 561
• Main Authors: Kozhevnikova, E. Yu, Petrova, D. A., Novikov, A. A., Shnyreva, A. V., Barkov, A. V., Vinokurov, V. A.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-09-2017; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Carboxymethyl cellulose; Carboxymethylcellulose; Cellulase; Cellulose; Crystalline cellulose; Direct conversion; Enzymatic activity; Enzyme activity; Ethanol; Fungi; Life Sciences; Lignocellulose; Medical Microbiology; Microbiology; Rye; Rye straw; Straw; Temperature effects; xylotrophic fungi; consolidated bioprocessing; cellulolytic activity; ethanol; lignocellulose
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683817050106

Sixty-six isolates of basidiomycete fungi were screened for the ability to synthesize cellulase. The effect of temperature on cellulase activity was studied for eight basidiomycete strains as perspective producers of ethanol. The temperature optima of enzyme activity ranged between 26 and 32°C. Direct conversion of Na-carboxymethyl cellulose, microcrystalline cellulose and rye straw were studied for seven basidiomycetes strains:
Fomitopsis pinicola
MT-5.09,
F. pinicola
MT-5.21,
Piptoporus betulinus
MT-30.04,
Fomes fomentarius
MT-4.05,
F. fomentarius
MT-4.23,
Trametes hirsuta
MT-24.24,
Flammulina velutipes
MT-3.03 Maximum ethanol production from Na-carboxymethyl cellulose (1.3 g/dm
3
) was achieved by strain
F. velutipes
MT-3.03. Strain
F. fomentarius
MT-4.05 more effectively converted rye straw to ethanol with yield of 1.1 g/dm
3
.