Genome-Wide Classification of Myb Domain-Containing Protein Families in Entamoeba invadens

• Published in: Genes Vol. 15; no. 2; p. 201
• Main Authors: Cuellar, Patricia, Castañeda-Ortiz, Elizabeth J, Rosales-Zarza, César, Martínez-Rodríguez, Carlos E, Canela-Pérez, Israel, Rodríguez, Mario Alberto, Valdés, Jesús, Azuara-Liceaga, Elisa
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02-02-2024
• Subjects: Amebiasis; Amino acids; Cell cycle; Chromatin remodeling; Cysts; Entamoeba - genetics; Entamoeba - metabolism; Entamoeba histolytica - genetics; Entamoeba invadens; Gene expression; Gene Expression Profiling; Gene Expression Regulation; Gene regulation; Genomes; Humans; Life cycles; Meiosis; Molecular modelling; MYB protein; Ontology; Parasitic diseases; Phylogenetics; Protein families; Protein transport; Proteins; Protozoa; Software; Telomeres; Transcription factors; Transcriptomics; Ubiquitin; protozoan; Myb recognition element; MYB-DBD-containing proteins; encystation–excystation; transcriptional regulation
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes15020201

, the causative agent of amebiasis, is the third leading cause of death among parasitic diseases globally. Its life cycle includes encystation, which has been mostly studied in
, responsible for reptilian amebiasis. However, the molecular mechanisms underlying this process are not fully understood. Therefore, we focused on the identification and characterization of Myb proteins, which regulate the expression of encystation-related genes in various protozoan parasites. Through bioinformatic analysis, we identified 48 genes in
encoding MYB-domain-containing proteins. These were classified into single-repeat 1R (20), 2R-MYB proteins (27), and one 4R-MYB protein. The in-silico analysis suggests that these proteins are multifunctional, participating in transcriptional regulation, chromatin remodeling, telomere maintenance, and
. Transcriptomic data analysis revealed expression signatures of
genes, suggesting a potential orchestration in the regulation of early and late encystation-excystation genes. Furthermore, we identified probable target genes associated with reproduction, the meiotic cell cycle, ubiquitin-dependent protein catabolism, and endosomal transport. In conclusion, our findings suggest that
Myb proteins regulate stage-specific proteins and a wide array of cellular processes. This study provides a foundation for further exploration of the molecular mechanisms governing encystation and unveils potential targets for therapeutic intervention in amebiasis.