Antioxidant capacity and cadmium accumulation in parsley seedlings exposed to cadmium stress

• Published in: Russian journal of plant physiology Vol. 64; no. 6; pp. 883 - 888
• Main Authors: Ulusu, Y., Öztürk, L., Elmastaş, M.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-11-2017; Springer Nature B.V
• Subjects: Accumulation; Antioxidants; Ascorbic acid; Biomedical and Life Sciences; Cadmium; Catalase; Chlorophyll; Contamination; Controlled conditions; L-Ascorbate peroxidase; Leaves; Life Sciences; Lipid peroxidation; Peroxidase; Peroxidation; Phenolic compounds; Phenols; Plant Physiology; Plant Sciences; Research Papers; Seedlings; Soil contamination; Soil pollution; Superoxide dismutase; ascorbate peroxidase; superoxide dismutase; polyphenol contents; cadmium; antioxidant activity; catalase; photosynthetic pigment
• ISSN: 1021-4437; 1608-3407
• DOI: 10.1134/S1021443717060139

Parsley (
Petroselinum hortense
L.) plants cultivated under controlled conditions were exposed to different doses of cadmium to investigate the antioxidant capacity and cadmium accumulation in the samples. Two-months-old parsley seedlings were treated with four different concentrations of CdCl
2
(0, 75, 150, and 300 μM) for fifteen days. Cadmium level in leaves increased significantly by increasing the Cd contamination in the soil. Total chlorophyll and carotenoid content declined considerably with Cd concentration. Cd treatment caused a significant increase lipid peroxidation in tissue of leaf. Superoxide dismutase activity (SOD, EC 1.15.1.1) increased partially at 75 and 150 μM CdCl
2
concentrations whereas the activity decreased at 300 μM CdCl
2
. Catalase (CAT, EC 1.11.1.6) and ascorbate peroxidase (APX, EC 1.11.1.11) activities were reduced by Cd application. Total phenolic compound amount increased significantly with increasing Cd concentration, as ferric reduction power, superoxide anion radical, and DPPH˙ free radical scavenging activities elevated slightly by the concentration. These results suggest that antioxidant enzymes activities were repressed depending on accumulation of cadmium in leaves of parsley while the non-enzymatic antioxidant activities slightly increased.