The enzymes of a marine bacterial isolate from the brown alga Sargassum polycystum agardh, 1821, that catalyzes the transformation of polyanionic oligo-and polysaccharides

• Published in: Russian journal of marine biology Vol. 43; no. 5; pp. 392 - 399
• Main Authors: Dubrovskaya, Yu. V., Kurilenko, V. V., Hang, Cao Thi Thuy, Ly, Bui Minh, Bakunina, I. Yu, Zvyagintseva, T. N., Mikhailov, V. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-09-2017; Springer Nature B.V
• Subjects: Algae; Alginate lyase; Alginic acid; Bacteria; Biochemical characteristics; Biochemistry; Biodegradation; Biomedical and Life Sciences; Biosynthesis; Composition; Enzymes; Epiphytes; Freshwater & Marine Ecology; Hydrolases; Life Sciences; Lyases; Microbiological strains; Microbiology; Microflora; pH effects; Physical characteristics; Polysaccharides; Saccharides; Sargassum polycystum; Seaweed meal; Strains (organisms); Temperature; Territorial waters; Thallus; alginate lyase; polymannuronate; genus; fucoidanase; marine bacteria; alginic acid; brown algae
• ISSN: 1063-0740; 1608-3377
• DOI: 10.1134/S1063074017050030

A search for enzymes involved in the degradation of polyanionic polysaccharides (fucoidans and alginic acid) was conducted among bacterial epiphytes of the brown alga
Sargassum polycystum
that grows in the territorial waters of the Socialist Republic of Vietnam. Two resistant bacterial strains, F10 and F14, have been isolated from the algal microflora that degrade the thallus of the alga under laboratory conditions. These bacterial strains differed in the morphological, physiological, and biochemical characteristics and in the composition of enzymes. The strains were studied for the ability to synthesize intracellular oligo-and polysaccharide hydrolases and alginate lyases. The optimal conditions for the growth of bacterial strain F14 and the biosynthesis of fucoidanase and polymannuronate-specific alginate lyase were determined. The partially purified alginate lyase was stable at a temperature up to 40°C and had an optimal pH 6.0 and an optimal temperature 35°C.