Methyl Jasmonate and Nitric Oxide in Regulation of the Stomatal Apparatus of Arabidopsis thaliana

• Published in: Cytology and genetics Vol. 52; no. 6; pp. 400 - 405
• Main Authors: Yastreb, T. O., Kolupaev, Yu. E., Kokorev, A. I., Horielova, E. I., Dmitriev, A. P.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-11-2018; Springer Nature B.V
• Subjects: Antagonists; Arabidopsis thaliana; Arginine; Biomedical and Life Sciences; Biomedicine; Epidermis; Human Genetics; Leaves; Methyl jasmonate; NG-Nitroarginine methyl ester; Nitrate reductase; Nitric oxide; Nitric-oxide synthase; Nitrites; Oxidation; Sodium; Sodium nitrite; Sodium tungstate; Stomata; stomata; nitric oxide; methyl jasmonate; nitrite; signaling; L-arginine
• ISSN: 0095-4527; 1934-9440
• DOI: 10.3103/S0095452718060129

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The effect of methyl jasmonate, donors and antagonists of nitric oxide (NO) on the state of stomata of
Arabidopsis thaliana
plants (
Col-0
) was studied. Treatment of the epidermis of leaves with a 50–400 μM solution of methyl jasmonate (MJ) caused a decrease in the size of the stomatal aperture and the percentage of open stomata. These effects were completely eliminated after preliminary treatment of epidermis cells with NO scavenger PTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) and partially after treatment with inhibitors of animal NO synthase (N
G
-nitro-L-arginine methyl ester, L-NAME) and nitrate reductase (sodium tungstate). NO donors (L-arginine and sodium nitrite) in concentrations of 0.5–2.0 mM also caused a decrease in the number of open stomata and the size of the stomatal aperture. The action of L‑arginine on the state of stomata was leveled by pretreatment of cells with L-NAME, and effects of sodium nitrite were eliminated by preaction of sodium tungstate. A conclusion about the possible participation of nitrogen oxide, forming both as the result of L-arginine oxidation and during nitrate/nitrite reduction, in the regulation of the stomatal apparatus and about its role in the realization of an MJ effect on state of stomata in
Arabidopsis thaliana
was made.