Development and Validation of a Simple Ultra Fast Liquid Chromatographic Method for the Simultaneous Determination of Aspartame, Acesulfame-K, Caffeine and Sodium Benzoate in Dietic Soft Drinks

• Published in: Journal of analytical chemistry (New York, N.Y.) Vol. 74; no. 6; pp. 555 - 564
• Main Authors: Üstün Özgür, M., Kasapoğlu, M.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-06-2019; Springer Nature B.V
• Subjects: Acetonitrile; Analytical Chemistry; Aspartame; Caffeine; Chemistry; Chemistry and Materials Science; Chromatography; Flow velocity; Ions; Sodium benzoate; Soft drinks; sodium benzoate; caffeine; acesulfame-K; aspartame; dietic soft drink; food additives; ultra fast liquid chromatography; determination; validation
• ISSN: 1061-9348; 1608-3199
• DOI: 10.1134/S1061934819060133

A rapid, specific, and sensitive ultra fast liquid chromatographic method for simultaneous determination of aspartame (
ASP
), acesulfame-K (
ACE-K
), caffeine (
CAF
) and sodium benzoate (
SB
) has been developed and validated. The chromatographic separation was achieved on a Shim-pack XR-ODS column within a run time of 3 min using acetonitrile‒pH 3 phosphate buffer (20 : 80, v/v) as the mobile phase at a flow rate 1.5 mL/min and the column temperature was set at 40°C. The elaborated method was validated according to the International Conference on Harmonization quidelines. Linear calibration graphs were in the ranges of 120‒280 µg/mL for ASP and ACE-K, 90‒210 µg/mL for CAF and 60‒140 µg/mL for SB. The limits of detection were 1.6, 4.3, 3.9 and 4.1 µg/mL for ASP, ACE-K, CAF and SB, respectively. The limits of quantitation were 5.2, 14.4, 12.9 and 13.7 µg/mL for ASP, ACE-K, CAF and SB, respectively. The intra- and inter-day precisions were less than 2%. The recoveries for the analytes were between 98.87‒99.73% and the precision values from 0.06 to 0.35.