A novice Achromobacter sp. EMCC1936 strain acts as a plant-growth-promoting agent

• Published in: Acta physiologiae plantarum Vol. 39; no. 2; pp. 1 - 15
• Main Authors: Abdel-Rahman, H. M., Salem, A. A., Moustafa, Mahmoud M. A., El-Garhy, Hoda A. S.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-02-2017; Springer Nature B.V
• Subjects: Acetic acid; Achromobacter; Agriculture; Azotobacter chroococcum; Bacteria; Biochemical characteristics; Biochemistry; Biomedical and Life Sciences; Enzymatic activity; Gene sequencing; Greenhouses; In vitro methods and tests; Indoleacetic acid; Inoculation; Life Sciences; Nitrogenase; Original Article; Phosphates; Plant Anatomy/Development; Plant Biochemistry; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Rock phosphate; rRNA 16S; Soils; Tomatoes; Virulence; Virulence factors; Transmission (TEM); Sustainable agriculture; gene; Scanning electron microscopy (SEM); Plant-growth-promoting rhizobacteria (PGPR); sp
• ISSN: 0137-5881; 1861-1664
• DOI: 10.1007/s11738-017-2360-6

Fifteen bacterial isolates were isolated from a watering canal at Al Hadady-Damrou, Kafr El-Sheikh Governorate, Egypt (31.3°N 30.93°E). The screening process was achieved based on nitrogenase activity. The most potent bacterial isolate (B9) was tested as plant-growth-promoting rhizobacteria (PGPR). Ultrastructural, cultural, biochemical characteristics and
16S rDNA
partial sequence were used for the isolate identification and characterization. From the
16S rRNA
gene sequencing results, the nearest bacterial species to our isolate was
Achromobacter marplatensis
B2 (T), EU150134.1, with 97% matching. The sequence was submitted to the NCBI website with the accession number GenBank: KM491552.1. In vitro analysis revealed that the isolate under study is non-pathogenic (virulence factors-free) and capable of producing indole acetic acid (IAA), gibberellin (GA3) and solubilizing rock phosphate. Under greenhouse conditions, tomato inoculation with the obtained
Achromobacter
sp. EMCC1936 significantly increased vegetative growth, yield parameters and endogenous phytohormones content as compared with common free diazotrophic PGPR,
Azotobacter chroococcum
EMCCN1458. It was deposited in Microbiological Resource Center for public use with number (EMCC1936). Data revealed the importance of soil inoculation with the obtained isolate and of its role in increasing soil enzymatic activity. These features fulfill the isolate to be used as a PGPR for various crops.