Polarization-shaped laser pulses for improved fluorescence anisotropy contrast

• Published in: The European physical journal. D, Atomic, molecular, and optical physics Vol. 67; no. 8
• Main Authors: Achazi, Georg, Hermes, Nils, Patas, Alexander, Tolksdorf, Daniel, Lindinger, Albrecht
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-08-2013; EDP Sciences
• Subjects: Applications of Nonlinear Dynamics and Chaos Theory; Atomic; Atomic and molecular physics; Exact sciences and technology; Fluorescence and phosphorescence spectra; Fluorescence and phosphorescence; radiationless transitions, quenching (intersystem crossing, internal conversion); Molecular; Molecular properties and interactions with photons; Optical and Plasma Physics; Physical Chemistry; Physics; Physics and Astronomy; Quantum Information Technology; Quantum Physics; Regular Article; Spectroscopy/Spectrometry; Spintronics; Optical Phenomena and Photonics; Fluorescence spectrum; Organic dye; Laser pulse; Emission spectra; Absorption spectra; Xanthene dye; Stilbene derivatives; Organic compounds; Stilbene
• ISSN: 1434-6060; 1434-6079
• DOI: 10.1140/epjd/e2013-30758-5

We present a method to change the fluorescence contrast between two dyes with high fluorescence anisotropy in the same solution under different polarization directions by using polarization-shaped pulses. First a theoretical relationship between contrast, anisotropy and polarization is derived for this case. Then the wavelength dependent polarization anisotropy and the change of contrast by phase-shaping for a solution of Rhodamine B and Stilbene 3 in glycerol is shown. Finally, the application of the polarization-shaped pulses is demonstrated.