Characteristics of fungal strains producing thermostable xyloglucanases from the Russian National Collection of industrial microorganisms

• Published in: Molecular genetics, microbiology and virology Vol. 31; no. 3; pp. 149 - 155
• Main Authors: Krestyanova, I. N., Sakhibgaraeva, L. F., Berezina, O. V., Rykov, S. V., Zavyalov, A. V., Zverlov, V. V., Yarotsky, S. V.
• Format: Journal Article
• Language: English
• Published: New York Allerton Press 01-07-2016; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Experimental Works; Fungi; Life Sciences; Microbiology; Molecular Medicine; Mycology; enzymatic hydrolysis; plant biomass; xyloglucan; xyloglucanase
• ISSN: 0891-4168; 1934-841X
• DOI: 10.3103/S089141681603006X

Fungal strains possessing xyloglucanase activity have been selected from the microorganisms degrading plant biomass deposited in the Russian National Collection of Industrial Microorganisms (VKPM). The thermophilic strains
Sporotrichum thermophile
VKPM F-972,
Myceliophthora thermophila
VKPM F-244, and
Sporotrichum pruinosum
VKPM F-235 produced extracellular xyloglucanases under conditions of submerged cultivation with optimum temperature of 60°C and pH of 5.0. The enzymes retained 88–100 and 79–84% of the initial activity after 1-h incubation at 50° and 60°C, respectively. Thus, strains
S. thermophile
VKPM F-972,
M. thermophila
VKPM F-244, and
S. pruinosum
VKPM F-235 may be used as sources of genes for construction of highly active producers of thermostable xyloglucanases.