EphB3 receptor suppressor invasion, migration and proliferation in glioma by inhibiting EGFR-PI3K/AKT signaling pathway

• Published in: Brain research Vol. 1830; p. 148796
• Main Authors: Xiao, Zumu, Huang, Shengxuan, Qiu, Wenjin, Pang, Mengru, Zeng, Xi, Xu, Xu, Yang, Yushi, Yang, Binglin, Chu, Liangzhao
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-05-2024
• Subjects: EphB3 receptor; Glioma; Invasion; Migration; PI3K/AKT; Proliferation; Proliferation; Glioma; Migration; Invasion; EphB3 receptor; PI3K/AKT
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2024.148796

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•EphB3 receptor is downregulated in glioma tissues and cell lines with increasing tumor grade.•Knockdown of EphB3 gene enhances invasion, migration, and proliferation of glioma cells in vitro.•EphB3 overexpression reduces glioma cell invasion, growth, and downregulates EGFR/PI3K/AKT pathway.
Eph receptors are the largest subfamily of receptor tyrosine kinases, and they have been shown to play a crucial role in glioma. The EphB3 receptor is a member of this family, and its effect on the invasion, migration and proliferation of glioma cells was examined in this study. It was found that the expression of EphB3 was decreased in glioma specimens with increasing tumor grade. Additionally, the U87MG and U251 cell lines showed low levels of EphB3 expression. This finding was consistent with the negative correlation between EphB3 expression in glioma tissues and tumor grade.
Depletion of EphB3 gene in U87MG and U251 cell lines resulted in a substantial enhancement of their invasion, migration, and proliferation capacities in vitro. Furthermore, the knockdown of EphB3 led to an upregulation of EGFR, p-PI3K, and p-AKT protein levels. On the other hand, EphB3 overexpression reduced the invasiveness, proliferative capacity and migration rate of U87MG and U251 cells, and downregulated EGFR, p-PI3K and p-AKT. These findings indicate that EphB3 functions as a tumor suppressor in glioma, and its downregulation enhances the malignant potential of glioma cells by activating the EGFR-PI3K/AKT pathway. Thus, EphB3 is a promising diagnostic marker for glioma, and the EphB3-EGFR-PI3K / AKT axis deserves further investigation as a potential therapeutic target.