Mutation Induction by Different Dose Rates of Gamma Rays in Near-Diploid Mouse Cells in Plateau- and Log-Phase Culture

• Published in: Radiation research Vol. 136; no. 1; pp. 97 - 102
• Main Authors: Furuno-Fukushi, Ikuko, Aoki, Kazuko, Matsudaira, Hiromichi
• Format: Journal Article
• Language: English
• Published: Oak Brook, Il Academic Press, Inc 01-10-1993; Radiation Research Society
• Subjects: Animals; Biological and medical sciences; Biological effects of radiation; Cell culture techniques; Cell Division; Cell growth; Cell lines; Cell Survival - radiation effects; Cells, Cultured; Cultured cells; Embryonic cells; Flasks; Fundamental and applied biological sciences. Psychology; Gamma Rays; Genetic mutation; Hypoxanthine Phosphoribosyltransferase - genetics; Interphase; Ionizing radiations; Irradiation; Mice; Mutagenesis; Mutation; Radiation damage; Radiation Dosage; Tissues, organs and organisms biophysics; Growing phase; Cell culture; Rodentia; Biophysics; Dose rate; Dose activity relation; Vertebrata; Ionizing irradiation; Mammalia; Radiobiology; Mouse; Animal; Biological effect; Mutation; Cell
• ISSN: 0033-7587; 1938-5404
• DOI: 10.2307/3578645

Induction of mutation to 6-thioguanine resistance was studied in cultured near-diploid mouse cells (m5S) in plateau and log phase after exposure to γ rays at dose rates of 30 Gy/h, 180 mGy/h, or 13 mGy/h. In plateau-phase culture, lowering the dose rate from 30 Gy/h to 13 mGy/h resulted in an increase in cell survival and a marked decrease in induced mutation frequency. On the other hand, in the log-phase culture, the magnitude of the dose-rate effects was not as marked as in the plateau-phase culture, particularly within a dose range below 5 Gy. These results, together with those indicating the inverse dose-rate effects in growing mouse leukemia cells (Radiat Res. 115, 273-280, 1988), demonstrate the significant influence of cell growth that takes place during protracted irradiation, particularly for the induction of mutation.