Maintained upregulation of pulmonary eNOS gene and protein expression during recovery from chronic hypoxia

• Published in: The American journal of physiology Vol. 276; no. 2; pp. H699 - H708
• Main Authors: Resta, T C, Chicoine, L G, Omdahl, J L, Walker, B R
• Format: Journal Article
• Language: English
• Published: United States 01-02-1999
• Subjects: 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid - pharmacology; Animals; Chronic Disease; Gases - blood; Gene Expression Regulation - physiology; Hematocrit; Hemodynamics - physiology; Hypertrophy, Right Ventricular - etiology; Hypoxia - complications; Hypoxia - enzymology; Hypoxia - genetics; Hypoxia - physiopathology; In Vitro Techniques; Ionomycin - pharmacology; Lung - enzymology; Male; Nitric Oxide Synthase - genetics; Nitric Oxide Synthase - metabolism; Nitric Oxide Synthase Type III; Pulmonary Circulation - drug effects; Pulmonary Circulation - physiology; Rats; Rats, Sprague-Dawley; Vascular Resistance - drug effects; Vascular Resistance - physiology
• ISSN: 0002-9513
• DOI: 10.1152/ajpheart.1999.276.2.h699

We previously demonstrated augmented endothelium-derived nitric oxide (EDNO)-dependent pulmonary arterial dilation and increased arterial endothelial nitric oxide synthase (eNOS) levels in chronic hypoxic (CH) and monocrotaline (nonhypoxic) models of pulmonary arterial hypertension. Therefore, we hypothesized that the long-term elevation of arterial eNOS levels associated with CH is related to pulmonary hypertension or some factor(s) associated with hypertension and not directly to hypoxia. To test this hypothesis, we examined responses to the EDNO-dependent dilator ionomycin in U-46619-constricted, isolated, saline-perfused lungs from control rats, CH (4 wk at 380 mmHg) rats, and rats previously exposed to CH but returned to normoxia for 4 days or 2 wk. Microvascular pressure was assessed by double-occlusion technique, allowing calculation of segmental resistances. In addition, vascular eNOS immunoreactivity was assessed by quantitative immunohistochemistry, and eNOS mRNA abundance was determined by RT-PCR assays. Our findings indicate that 4-day and 2-wk posthypoxic rats exhibit persistent pulmonary hypertension, likely due to maintained arterial remodeling and polycythemia associated with prior exposure to CH. Furthermore, arterial dilation to ionomycin was augmented in lungs from each experimental group compared with controls. Finally, arterial eNOS immunoreactivity and whole lung eNOS mRNA levels remained elevated in posthypoxic animals. These findings suggest that altered vascular mechanical forces or vascular remodeling contributes to enhanced EDNO-dependent arterial dilation and upregulation of arterial eNOS in various models of established pulmonary hypertension.