Agrobacterium-mediated transformation of pepino and regeneration of transgenic plants

Regeneration of pepino (Solanum muricatum Ait.) shoots was achieved both by organogenesis and by embryogenesis. Shoots derived via organogenesis were easily rooted and most regenerated plants appeared phenotypically normal. Transgenic plants were obtained using the binary vector pKIWI110 in the avir...

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Bibliographic Details
Published in:	Plant cell reports Vol. 10; no. 4; pp. 208 - 212
Main Authors:	ATKINSON, R. G, GARDNER, R. C
Format:	Journal Article
Language:	English
Published:	Berlin Springer 01-07-1991
Subjects:	Biological and medical sciences Biotechnology Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Plant cells and fungal cells Protoplast preparation, cell fusion and regeneration Regeneration Transgenic plant Genetic transformation Experimental protocol Vector
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Summary:	Regeneration of pepino (Solanum muricatum Ait.) shoots was achieved both by organogenesis and by embryogenesis. Shoots derived via organogenesis were easily rooted and most regenerated plants appeared phenotypically normal. Transgenic plants were obtained using the binary vector pKIWI110 in the avirulent Agrobacterium tumefaciens strain LBA4404. Optimization of transformation protocols was rapidly achieved by monitoring early expression of the GUS (β-D-glucuronidase) reporter gene carried on pKIWI110. Transgenic plants expressed GUS and selectable marker genes for kanamycin resistance and chlorsulfuron resistance. PCR (polymerase chain reaction) and Southern analysis provided molecular evidence for transformation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0721-7714 1432-203X
DOI:	10.1007/BF00234297