Countercurrent Chromatographic Purification of Polysaccharides from Achyranthes bidentata with an Aqueous Two-Phase System Using a Cross-Axis Coil Planet Centrifuge

• Published in: Journal of liquid chromatography & related technologies Vol. 26; no. 12; pp. 1895 - 1903
• Main Authors: Chao, Zhimao, Shibusawa, Yoichi, Shindo, Heisaburo, Ito, Yoichiro
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 01-01-2003; Taylor & Francis
• Subjects: Biological and medical sciences; Countercurrent chromatography; Cross-axis coil planet centrifuge; General pharmacology; Medical sciences; Partition coefficient; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Polysaccharides; Asia; China; Countercurrent chromatography; Purification; Root; Preparative chromatography; Amaranthaceae; Medicinal plant; Separation method; Folk medicine; Dicotyledones; Angiospermae; Plant origin; Isolation; Spermatophyta; Polysaccharide
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1081/JLC-120021758

Polysaccharides were purified from a high molecular weight complex of Achyranthes bidentata Bl. (Fam. Amaranthaceae) root by high speed countercurrent chromatography (HSCCC) using a cross-axis coil planet centrifuge (X-axis CPC). The purification was performed with an aqueous polymer two-phase system composed of 12.5% (w/w) polyethylene glycol (PEG) 1000, 8% (w/w) potassium dihydrogen phosphate, and 8% (w/w) dipotassium hydrogen phosphate buffer at pH 6.8. The preparative separation of the crude sample (2 g) was successfully performed, yielding 1.1 g of polysaccharides at high purity.