Assessment of Virulence Potential and Antibiotic Resistance Profiles in E. coli Isolates from Selected Ground Water Samples Around the Control Open Dump Sites in Sri Lanka

• Published in: Waste and biomass valorization Vol. 15; no. 9; pp. 5475 - 5485
• Main Authors: Liyanage, G. Y., Wijerathna, P. A. K. C., Bandara, S. M. T. V., Manage, Pathmalal M.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 2024; Springer Nature B.V
• Subjects: Antibiotic resistance; Antibiotics; Bioaccumulation; Colitis; Contamination; Drug resistance; E coli; Engineering; Environment; Environmental Engineering/Biotechnology; Groundwater pollution; Hemolytic uremic syndrome; Industrial Pollution Prevention; Intimin; Leachates; Original Paper; Percolation; Renewable and Green Energy; Shiga toxin; Soil contamination; Solid wastes; Thrombocytopenic purpura; Thrombotic thrombocytopenic purpura; Toxins; Urbanization; Virulence; Waste Management/Waste Technology; Water analysis; Water sampling; Sri Lanka; Groundwater contamination; (; ); Leachate; Municipal solid waste; Antibiotic resistance
• ISSN: 1877-2641; 1877-265X
• DOI: 10.1007/s12649-024-02544-x

  
Urbanization and accelerated industrialization have led to significant waste generation following the accumulation of massive amounts of solid waste in open dump sites. Groundwater contamination is one of the critical ecological concerns associated with the percolation of leachate from dump sites.
E.coli
O157 is a particular virulent serotype which produces Intimin and Shiga toxins, that cause severe diseases including Hemorrhagic Colitis, Hemolytic Uremic Syndrome and Thrombotic Thrombocytopenic Purpura in humans. The focus of the present study is to study the virulence potential and antibiotic resistance profiles in
E. coli
isolates from selected Karadiyana, Methotamulla and Kerawalapitiya control open dump sites in Sri Lanka. The
E.coli
was isolated following the standard MPN method and 5 randomly selected ( n = 5) colonies from each location were subjected to the virulence potential tests and antibiotic resistivity study. The results showed the total coliform count was ranged from 0–120 MPN/mL around the Kardiyana dump site whereas 0–75 MPN/mL and 3–115 MPN/mL recorded in the Methotamulla and the Kerawalapitiya dump sites. Overall, resistance in isolated
E.coli
against AMX, AMP, SUF/ TRI, SDI, CLOX, TET and ERM was high (> 70%) compared with the other tested antibiotics namely CIP, GEN and AZY (< 40%). According to the results, the Enteropathogenic
E. coli
pathotype was identified in 17 samples, whereas the Enterohaemorrhagic
E. coli
pathotype was found in only 3 samples.
Graphical Abstract