A specific radioimmunoassay for human beta-lipotropin

A specific radioimmunoassay for human beta-lipotropin

A homologous RIA for human beta-lipotropin (beta hLPH) has been developed. At a final dilution of 1:24,000, the antiserum employed shows cross-reaction with beta hLPH but none with human beta-MSH (beta hMSH), and it is concluded that the antigenic determinant lies within the N-terminal 1-36 region o...

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Bibliographic Details
Published in:The journal of clinical endocrinology and metabolism Vol. 47; no. 1; p. 160
Main Authors: Jeffcoate, W J, Rees, L H, Lowry, P J, Besser, G M
Format: Journal Article
Language:English
Published: United States 01-07-1978
Subjects:
beta-Lipotropin - blood
Humans
Hydrocortisone - blood
Hypoglycemia - blood
Immune Sera
Insulin
Iodine Radioisotopes
Kinetics
Radioimmunoassay - methods
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Summary:A homologous RIA for human beta-lipotropin (beta hLPH) has been developed. At a final dilution of 1:24,000, the antiserum employed shows cross-reaction with beta hLPH but none with human beta-MSH (beta hMSH), and it is concluded that the antigenic determinant lies within the N-terminal 1-36 region of beta hLPH. With extraction of 3-ml plasma samples, the assay is sufficiently sensitive to measure circulating beta hLPH levels in normal individuals at 0900 h (25-200 pg/ml). There is a circadian variation with levels falling to (less than 20-80 pg/ml) at 2300 h. beta hLPH levels rise after metyrapone and after insulin-induced hypoglycemia, and fall after administration of dexamethasone. In patients with a variety of diseases of the pituitary-adrenal axis, levels of beta hLPH follow immunoreactive ACTH levels, although the two are not always secreted on a 1:1 molar basis.
ISSN:0021-972X
DOI:10.1210/jcem-47-1-160