Unusual sequence of an abscisic acid-inducible mRNA which accumulates late in Brassica napus seed development

• Published in: Plant molecular biology Vol. 12; no. 4; pp. 395 - 401
• Main Authors: Harada, J.J, DeLisle, A.J, Baden, C.S, Crouch, M.L
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01-04-1989
• Subjects: abscisic acid; amino acid sequences; Biological and medical sciences; Brassica napus; desiccation; embryo (plant); embryogenesis; Fundamental and applied biological sciences. Psychology; Gene expression; induction; late embryogenesis-abundant gene; lea76 gene; messenger RNA; Molecular and cellular biology; Molecular genetics; molecular sequence data; multigene family; nucleotide sequences; restriction mapping; seed development; seedlings; temporal variation; Seeds; Activation; Characterization; Proteins; Messenger RNA; Gene; Cruciferae; Dicotyledones; Blotting assay; Angiospermae; Abscisic acid; Multigene family; Molecular cloning; Aminoacid sequence; Quantitative analysis; Embryonic development; Vegetals; Nucleotide sequence; Induction; Germination; Gene expression; Ripening; Complementary DNA; Brassica napus; Spermatophyta
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/bf00017579

We have analyzed the nucleotide sequence and accumulation of an mRNA which is prevalent in seeds of Brassica napus L. During normal development, the mRNA begins to accumulate during late embryogeny, is stored in dry seeds, and becomes undetectable in seedlings within 24 hours after imbibition Moreover, abscisic acid treatment of embryos precociously induces or enhances accumulation of the mRNA. Nucleotide sequencing studies show that the deduced 3O kDa polypeptide has an unusual primary structure; the polypeptide possesses direct amino acid sequence repeats and is virtually entirely hydrophilic with the exception of a hydrophobic carboxyl-terminal region. Based upon the expression pattern and predicted polypeptide sequence, we conclude that the mRNA is encoded by a late embryo-genesis-abundant (Lea) gene in B. napus.