Water relations in silver birch during springtime: How is sap pressurised?

• Published in: Plant biology (Stuttgart, Germany) Vol. 20; no. 5; pp. 834 - 847
• Main Authors: Hölttä, T, Dominguez Carrasco, M D R, Salmon, Y, Aalto, J, Vanhatalo, A, Bäck, J, Lintunen, A
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01-09-2018
• Subjects: Birch trees; Carbohydrates; Diurnal; Embolism; Environmental changes; Environmental conditions; Flow rates; Flow velocity; Moisture content; Osmosis; Osmotic pressure; Parenchyma; Pressurization; Refilling; Soil water; Starch; Sugar; Thawing; Vessels; Water content; Water distribution; Water relations; Water transfer; Water uptake; Xylem; xylem transport; sap pressure; sap flow; Diameter change; silver birch
• ISSN: 1435-8603; 1438-8677
• DOI: 10.1111/plb.12838

Positive sap pressures are produced in the xylem of birch trees in boreal conditions during the time between the thawing of the soil and bud break. During this period, xylem embolisms accumulated during wintertime are refilled with water. The mechanism for xylem sap pressurization and its environmental drivers are not well known. We measured xylem sap flow, xylem sap pressure, xylem sap osmotic concentration, xylem and whole stem diameter changes, and stem and root non-structural carbohydrate concentrations, along with meteorological conditions at two sites in Finland during and after the sap pressurisation period. The diurnal dynamics of xylem sap pressure and sap flow during the sap pressurisation period varied, but were more often opposite to the diurnal pattern after bud burst, i.e. sap pressure increased and sap flow rate mostly decreased when temperature increased. Net conversion of soluble sugars to starch in the stem and roots occurred during the sap pressurisation period. Xylem sap osmotic pressure was small in comparison to total sap pressure, and it did not follow changes in environmental conditions or tree water relations. Based on these findings, we suggest that xylem sap pressurisation and embolism refilling occur gradually over a few weeks through water transfer from parenchyma cells to xylem vessels during daytime, and then the parenchyma are refilled mostly during nighttime by water uptake from soil. Possible drivers for water transfer from parenchyma cells to vessels are discussed. Also the functioning of thermal dissipation probes in conditions of changing stem water content is discussed.