Caproicibacter sp. BJN0012, a potential new species isolated from cellar mud for caproic acid production from glucose

• Published in: Journal of biotechnology Vol. 388; pp. 11 - 23
• Main Authors: Dai, Mengqi, Xu, Youqiang, Zhao, Lei, Wu, Mengqin, Ma, Huifeng, Zhu, Lining, Li, Weiwei, Li, Xiuting, Sun, Baoguo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 10-06-2024
• Subjects: Baijiu; Caproates - metabolism; Caproic acid; Fermentation; Genome, Bacterial - genetics; Glucose; Glucose - metabolism; Metabolic pathway; New species; Phylogeny; RNA, Ribosomal, 16S - genetics; Thiolase; New species; Metabolic pathway; Glucose; Baijiu; Caproic acid; Thiolase
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/j.jbiotec.2024.04.005

Acids play a crucial role in enhancing the flavor of strong-aroma Baijiu, and among them, caproic acid holds significant importance in determining the flavor of the final product. However, the metabolic synthesis of caproic acid during the production process of Baijiu has received limited attention, resulting in fluctuations in caproic acid content among fermentation batches and generating production instability. Acid-producing bacteria found in the cellar mud are the primary microorganisms responsible for caproic acid synthesis, but there is a lack of research on the related microbial resources and their metabolic properties. Therefore, it is essential to identify and investigate these acid-producing microorganisms from cellar mud to ensure stable caproic acid synthesis. In this study, a unique strain was isolated from the cellar mud, exhibiting a 98.12 % similarity in its 16 S rRNA sequence and an average nucleotide identity of 79.57 % with the reference specie, together with the DNA-DNA hybridization of 23.20 % similarity, confirming the distinct species boundaries. The strain was able to produce 1.22 ± 0.55 g/L caproic acid from glucose. Through genome sequencing, annotation, and bioinformatics analysis, the complete pathway of caproic acid synthesis from glucose was elucidated, and the catalytic mechanism of the key thiolase for caproic acid synthesis was investigated. These findings provide useful fundamental data for revealing the metabolic properties of caproic acid-producing bacteria found in cellar mud.
•A potential new species is isolated from cellar mud of Baijiu.•The species could produce caproic acid from glucose.•The possible key thiolase for caproic acid production is identified.•The work offers a valuable strain for producing caproic acid in Baijiu fermentation.