Spirulina (Arthrospira platensis) supplementation: Impact on growth, metabolism, and antioxidant status in zebrafish

• Published in: Journal of animal physiology and animal nutrition Vol. 108; no. 5; pp. 1189 - 1202
• Main Authors: Coli, Aline Pereira, Carneiro, William Franco, Silva, Kiara Cândido Duarte, Castro, Tássia Flávia Dias, Oliveira, João Paulo Lima, Martins, Moisés Silvestre Azevedo, Murgas, Luis David Solis
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01-09-2024
• Subjects: antioxidant enzyme activity; Antioxidants; Arthrospira platensis; Catalase; Cholesterol; Condition factor; Danio rerio; Diet; Dietary supplements; Enzymatic activity; Enzyme activity; Glutathione; growth performance; Juveniles; Lipid metabolism; Lipids; Maintenance costs; Medical research; Metabolism; microalgae; Nutrient deficiency; Nutritional requirements; Superoxide dismutase; Triglycerides; Zebrafish; antioxidant enzyme activity; microalgae; growth performance; Danio rerio
• ISSN: 0931-2439; 1439-0396
• DOI: 10.1111/jpn.13964

Zebrafish (Danio rerio) is a valuable model for biomedical research because of its mammalian genetic similarities, rapid reproduction, and low maintenance costs. However, further investigation is required regarding their nutritional requirements and standardized laboratory diets. This study evaluated the metabolic and growth responses of zebrafish juveniles fed on diets supplemented with spirulina, Arthrospira platensis (SP) at different levels for 77 days. Six diets with SP inclusion levels of 0%, 2%, 4%, 6%, 8%, and 10% (SP0‐SP10) were formulated. A total of 300 zebrafish juveniles with an average initial weight of 0.113 ± 0.10 g (mean ± SD) were randomly distributed across six groups, with five replicates per group, each containing 10 animals. After 77 days, the SP6 group demonstrated significantly enhanced growth performance compared with the other supplementation levels. The condition factor was markedly higher in the SP6 and SP8 groups than in the SP0 group. No significant effects on total cholesterol levels were observed, but the SP4, SP6, and SP10 diets decreased triglyceride levels. Lipase activity was higher in the SP6 and SP8 groups than in the control group, whereas amylase activity showed no significant differences between treatments. Catalase and superoxide dismutase activities were significantly higher in the SP8 and SP10 groups than in the SP0 and SP2 groups. Glutathione S‐transferase activity was higher in the SP6, SP8, and SP10 groups than in the SP0 group. In addition, SP inclusion in zebrafish diets improved female gonadal development. In conclusion, this study indicates that SP supplementation has substantial potential as a growth promoter, positively influencing lipid metabolism and antioxidant enzyme activity without affecting zebrafish survival.