Development of PCR‐based genetic test for detection of TTF1 mutation causing abortion in Holstein Friesian cattle

• Published in: Reproduction in domestic animals Vol. 59; no. 5; pp. e14581 - n/a
• Main Authors: Surati, Utsav, Niranjan, Saket Kumar
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01-05-2024
• Subjects: Abortion; Abortion, Veterinary - genetics; Animal populations; Animals; Cattle; Cattle - genetics; Cattle Diseases - diagnosis; Cattle Diseases - genetics; DNA-directed RNA polymerase; Female; Genetic screening; Genetic Testing - methods; Genetic Testing - veterinary; Holstein Friesian; Mutation; Nucleotides; PCR‐RFLP; Polymerase chain reaction; Polymerase Chain Reaction - methods; Polymerase Chain Reaction - veterinary; Polymorphism; Polymorphism, Restriction Fragment Length; Pregnancy; Restriction fragment length polymorphism; sequencing; Transcription Factors - genetics; Transcription termination; TTF1; abortion; TTF1; PCR‐RFLP; Holstein Friesian; sequencing
• ISSN: 0936-6768; 1439-0531
• DOI: 10.1111/rda.14581

A stop‐gain mutation (rs715966442; BTA11: 1,02,463,944 nucleotide position) in transcription termination factor, RNA polymerase I (TTF1) gene causes abortion in Holstein Friesian (HF) cattle. A PCR‐restriction fragment length polymorphism (PCR‐RFLP)‐based genetic test has been developed and validated to screen the TTF1 mutation locus in HF cattle. The mutation locus was screened in 80 HF and HF crossbreds using the protocol, which revealed two animals as carriers of the mutant TTF1 allele. The test employed is cost‐effective, rapid and precise and can be utilized as an effective tool for the screening of TTF1 mutation carriers in HF cattle population.