Biofilm formation by Salmonella Typhimurium and Staphylococcus aureus on stainless steel under either mono- or dual-species multi-strain conditions and resistance of sessile communities to sub-lethal chemical disinfection

• Published in: Food control Vol. 73; pp. 838 - 846
• Main Authors: Gkana, Eleni N., Giaouris, Efstathios D., Doulgeraki, Agapi I., Kathariou, Sophia, Nychas, George-John E.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-03-2017
• Subjects: Bacterial interactions; Biofilms; MRSA Staphylococcus aureus; Resistance; Salmonella Typhimurium; Sub-lethal chemical disinfection; Resistance; MRSA Staphylococcus aureus; Sub-lethal chemical disinfection; Biofilms; Bacterial interactions; Salmonella Typhimurium
• ISSN: 0956-7135; 1873-7129
• DOI: 10.1016/j.foodcont.2016.09.038

Intercellular interactions encountered within and between different bacterial species are believed to play key roles in both biofilm formation and antimicrobial resistance. In this study, Salmonella Typhimurium and Staphylococcus aureus (3 strains per species) were left to form biofilms on stainless steel coupons incubated at 20 °C for 144 h (i.e. 6 days), in periodically renewable growth medium, under either mono- or dual-species conditions. Subsequently, the developed sessile communities were exposed for 6 min to sub-lethal concentrations of: (i) benzalkonium chloride (BC, 50 ppm), (ii) sodium hypochlorite (NaClO, 10 ppm), or (iii) peroxyacetic acid (PAA, 10 ppm). The dominance of each strain in the mono- and dual-species biofilm communities, both before and after disinfection, was monitored by pulsed field gel electrophoresis (PFGE). Results revealed that dual-species conditions led to a significant (ca. 10-fold) reduction in the number of sessile cells for both species, compared to mono-species ones, with interspecies interactions however found to not exert any significant effect on the disinfection resistance of each species as a whole. However, PFGE analysis revealed that the different strains here employed behaved differently with regard to biofilm formation and disinfection resistance, with this effect to be also strongly dependent on the culture conditions (mono-/dual-species) and the disinfectant applied. Such results expand our knowledge on multi-species biofilms formed by foodborne pathogenic bacteria and could hopefully be helpful in our efforts to develop effective elimination strategies and thus improve food safety.
•S. enterica and S. aureus formed mono- and dual-species biofilms on stainless steel.•Both bacterial species formed less biofilm (ca. 10-fold) under co-culture.•Resistance of each species was not influenced by interspecies interactions.•Bacterial interactions influenced both dominance and resistance of each strain.•Inter-and intra-species interactions must be considered in the study of biofilms.