Determination of fatty acids in the main lipoprotein classes by capillary gas chromatography: BF3/methanol transesterification of lyophilized samples instead of Folch extraction gives higher yields

• Published in: Analytical biochemistry Vol. 198; no. 1; p. 184
• Main Authors: Sattler, W, Puhl, H, Hayn, M, Kostner, G M, Esterbauer, H
• Format: Journal Article
• Language: English
• Published: United States 01-10-1991
• Subjects: Blood Proteins - chemistry; Boranes - chemistry; Chromatography, Gas - methods; Esterification; Fatty Acids - analysis; Freeze Drying; Humans; Lipoprotein(a); Lipoproteins - chemistry; Lipoproteins, HDL - chemistry; Lipoproteins, LDL - chemistry; Lipoproteins, VLDL - chemistry; Methanol - chemistry
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(91)90526-y

The amount of individual fatty acids contained in the main human lipoproteins VLDL, LDL, lipoprotein (a), HDL2, and HDL3 were determined by two different methods. In Method I, the lipids were first extracted by the classical Folch procedure and then transesterified with BF3/methanol and separated by capillary GC. In Method II the lipoprotein solution was freeze dried prior to transesterification with BF3/methanol. In all lipoproteins except VLDL significantly more fatty acids were found with Method II as compared to Method I. For total fatty acids the increase was up to 17.5%, for polyunsaturated fatty acids up to 24.5%. The total fatty acid content determined by Method II resembled closely the content independently derived from the enzymatically determined lipid composition. The results indicate that in case of lipoproteins quantification of fatty acids should be made with freeze-dried samples rather than with Folch extracts.