Esteblishment and characterization of the line of Fagopyrum tataricum morphogenic callus tolerant to aminotriazole

• Published in: Russian journal of plant physiology Vol. 59; no. 5; pp. 662 - 669
• Main Authors: Sibgatullina, G. V, Rumyantseva, N. I, Khaertdinova, L. R, Akulov, A. N, Tarasova, N. B, Gumerova, E. A
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01-09-2012; SP MAIK Nauka/Interperiodica
• Subjects: Biomedical and Life Sciences; buckwheat; callus; catalase; death; Fagopyrum tataricum; hydrogen peroxide; Life Sciences; oxidative stress; Plant Physiology; Plant Sciences; Research Papers; viability; tolerance to aminotriazole; morphogenic callus; hydrogen peroxide; non-morphogenic callus; 3-amino-1,2,4-triazole; catalase; oxidative stress
• ISSN: 1021-4437; 1608-3407
• DOI: 10.1134/S1021443712050172

It is shown that the inhibitor of catalase 3-amino-1,2,4-triazole (AT) at the concentration of 2 mM affected differently growth of tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) callus lines differing in the morphogenecity. In some cases, AT induced the death of a great fraction of non-morphogenic callus cells; in other cases, it inhibited growth and reduced viability of morphogenic callus. The death of non-morphogenic callus cells may be related to the accumulation of hydrogen peroxide and the development of oxidative stress. After morphogenic callus treatment with AT, we obtained a modified line 1–8 AT tolerant to AT and differing from the original line in morphology, cell sizes, proliferative activity, and some biochemical characteristics. In the 1–8 AT line, catalase was sensitive to this inhibitor action. In this case, catalase inactivation with AT did not increase the content of hydrogen peroxide in the cells, which may indicate the compensatory functioning of another/others mechanism(s) destroying hydrogen peroxide.