The specific anti-biofilm effect of gallic acid on Staphylococcus aureus by regulating the expression of the ica operon

• Published in: Food control Vol. 73; pp. 613 - 618
• Main Authors: Liu, Meihui, Wu, Xiaoxia, Li, Jianke, Liu, Liu, Zhang, Runguang, Shao, Dongyan, Du, Xiaodan
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-03-2017
• Subjects: Anti-biofilm; Food industry; Gallic acid; ica operon; Staphylococcus aureus; Anti-biofilm; Gallic acid; ica operon; Food industry; Staphylococcus aureus
• ISSN: 0956-7135; 1873-7129
• DOI: 10.1016/j.foodcont.2016.09.015

Staphylococcus aureus (S. aureus) biofilms are of considerable interest in food safety because biofilms can increase the risk of food contamination and enhance the pathogenicity of bacteria. The ica-encoded polysaccharide intercellular adhesin (PIA) plays an important role in biofilm formation. In this study, the MIC of gallic acid against S. aureus in suspension and in biofilms was 2 mg/mL and 4 mg/mL, respectively. Quantitative crystal violet staining of biofilms showed that 2 mg/mL gallic acid can effectively inhibit biofilm formation and the ESEM images clearly showed the three-dimensional biofilm morphology of the S. aureus and the resulting anti-biofilm effect. The determination of viable bacteria in the biofilm revealed that gallic acid penetrated the biofilm to kill S. aureus, the bactericidal effect on the biofilm bacteria was comparable to that of planktonic bacteria. We further explored the influence of gallic acid on ica family gene expression and polysaccharide slime formation in S. aureus biofilm formation. The results showed that icaR was significantly activated that; icaA and icaD were downregulated in a dose-dependent manner with increasing concentrations of gallic acid; however, the expression of icaB and icaC was not significantly affected. The polysaccharide slime formation was reduced as well. Based on these results, gallic acid, as a natural substance, may play an important role in the food industry.
•Gallic acid can effectively inhibited S. aureus biofilm formation.•We studied the expression of five genes of the ica operon effected by gallic acid.•Gallic acid may potentially be used to control the food safety problems.