Investigation of the Effects of Isotretinoin on Spermatogenesis in Balb/c Mice

• Published in: Bezmialem science Vol. 8; no. 3; pp. 246 - 249
• Main Authors: KUMAŞ, Meltem, EŞREFOĞLU, Mukaddes
• Format: Journal Article
• Language: English
• Published: Istanbul Galenos Yayınevi 01-07-2020; Galenos Publishing House
• Subjects: Acids; balb/c mice; Conflicts of interest; Ethics; Experiments; follicle stimulating hormone; Gene expression; Hormones; isotretinoin; luteinizing hormone; Normal distribution; Spermatogenesis; Testosterone; Tıp; Vitamin A; İstanbul; Türkiye; United States > US; follikül stimüle edici hormon; Balb/c mice; testosterone; luteinize edici hormon; Balb/c fare; İzotretinoin; Isotretinoin; luteinizing hormone; follicle stimulating hormone; testosteron
• ISSN: 2148-2373; 2148-2373
• DOI: 10.14235/bas.galenos.2019.2542

Objective: It was aimed to investigate the effect of isotretinoin
(ISR), a retinoid derivative of vitamin A, on spermatogenesis via
follicle-stimulating hormone (FSH), luteinizing hormone (LH) and
testosterone levels.
Methods: Twelve male Balb/c mice were divided into two groups:
Control and ISR groups. FSH, LH and testosterone levels were
biochemically determined by ELISA assay. All statistical analyses
were made with SPSS 20.0 (IBM, New York, USA) and graphics
were prepared with Graph Pad Prism Version 6.01 (USA). The
parametric Student’s t-test was applied for comparison among
groups. A p value ≤0.05 was accepted as statistically significant.
Results: Mean FSH, LH and testosterone levels were increased
in the ISR group when compared to those of control group. The
increases in all hormone levels were found statistically significant
between the two groups (p<0.05).
Conclusion: The increase in serum levels of FSH, LH and
testosterone of the ISR group compared to the control group
suggests that a dose of 40 mg/kg of ISR may have stimulant effect
on spermatogenesis.