IDENTIFICATION, ISOLATION, AND DETERMINATION OF FLAVONES IN ORIGANUM VULGARE FROM MACEDONIAN FLORA

• Published in: Journal of liquid chromatography & related technologies Vol. 24; no. 4; pp. 589 - 600
• Main Authors: Kulevanova, Svetlana, Stefova, Marina, Stefkov, Gjoshe, Stafilov, Trajce
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 12-01-2001; Taylor & Francis
• Subjects: Biological and medical sciences; General pharmacology; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Europe; Macedonia; Chemical analysis; Pharmacognosy; Aglycone; HPLC chromatography; Oregano; Flavonoid; Medicinal plant; Thin layer chromatography; Reversed phase chromatography; Polyphenol; Ultraviolet detector; Folk medicine; Labiatae; Apigenin; Dicotyledones; Angiospermae; Plant origin; Flavoring crop; Spermatophyta; Qualitative analysis; Luteolin; Origanum vulgare; Quantitative analysis; Ultraviolet visible spectrometry
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1081/JLC-100103396

Assay of flavone aglycones in extracts of Origanum vulgare from Macedonia including identification and isolation is performed. Hydrolyzed extracts were analyzed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Three aglycones were identified and then isolated by column chromatography and TLC. Further TLC, HPLC, and UV/VIS spectrophotometric analysis showed that the isolated compounds are luteolin (the most abundant), apigenin, and diosmetin.
A new and rapid HPLC method was developed only for determination of the content of luteolin, which can serve as an appropriate criterion for evaluation of the flavonoid content in the drug. The accuracy of the method was proven by the method of standard additions, which gave satisfactory values for the recovery (98.2-100.3 %). The quantity of luteolin found in the drug ranged from 0.40-0.50 % (m/m).