Development of broad-specific monoclonal antibody-based immunoassays for simultaneous ochratoxin screening in medicinal and edible herbs

• Published in: Food control Vol. 148; p. 109626
• Main Authors: Guo, Mengyue, zhang, Jing, Lv, Jianxin, Ke, Tongwei, Tian, Jiao, Miao, Kun, Wang, Yudan, Kong, Dandan, Ruan, Haonan, Luo, Jiaoyang, Yang, Meihua
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-06-2023
• Subjects: ic-ELISA; LFIA; Medicinal and edible herbs; Monoclonal antibody; Ochratoxins; LFIA; Medicinal and edible herbs; ic-ELISA; Ochratoxins; Monoclonal antibody
• ISSN: 0956-7135; 1873-7129
• DOI: 10.1016/j.foodcont.2023.109626

The frequent occurrence of ochratoxins in food and herbs poses a considerable threat to consumer and animal health. Here, we generated a broad-specificity monoclonal antibody against three ochratoxins. Concentrations for 50% inhibition (IC50) for OTA, OTB, and OTC were 0.37, 0.23, and 2.24 ng/mL, respectively. In the two matrices, the proposed indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) exhibited a limit of detection (LOD) of 1.5 μg/kg for OTA and 1.0 μg/kg for OTB, respectively, whereas the cut-off levels of lateral flow immunoassay (LFIA) for OTA and OTB were respectively determined to be 1 and 0.25 ng/mL. As for OTC, the LOD of ic-ELISA and cut-off concentration of LFIA were 15.5 μg/kg and 8 ng/mL for milkvetch root and 37.5 μg/kg and 16 ng/mL for ginger, respectively. The real sample detection results by ic-ELISA and LFIA methods were validated via LC-MS/MS analysis. Therefore, the developed immunoassays are useful and effective tools for high-throughput screening and rapid on-site determination of ochratoxins in complex matrices.
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•A broad-specific monoclonal antibody against three ochratoxins was prepared.•The IC50 values of the mAb were 0.37, 0.23, and 2.24 ng/mL for OTA, OTB, and OTC.•Two immunoassays were developed to detect ochratoxins in two medicinal and edible herbs.•OTA and OTB were detected in 20% of milkvetch root samples.•The amount of OTA in milkvetch root samples was as high as 205.3 μg/kg.