Histochemical methods for detection of heavy metals and strontium in the tissues of higher plants

Elaboration and application of histochemical methods for detection of heavy metals (Cd, Pb, Ni, Zn) and strontium for the purpose of investigating their distribution, accumulation, and translocation within the tissues of higher plants are discussed. Detailed protocols of metal detection with metallo...

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Bibliographic Details
Published in:	Russian journal of plant physiology Vol. 58; no. 4; pp. 721 - 727
Main Authors:	Seregin, I. V, Kozhevnikova, A. D
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer-Verlag 01-07-2011 SP MAIK Nauka/Interperiodica
Subjects:	Biomedical and Life Sciences cadmium fluorescence microscopy heavy metals lead Life Sciences Methods nickel Plant Physiology Plant Sciences sodium strontium zinc histochemical methods nickel higher plants strontium cadmium zinc lead
Online Access:	Get full text
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Summary:	Elaboration and application of histochemical methods for detection of heavy metals (Cd, Pb, Ni, Zn) and strontium for the purpose of investigating their distribution, accumulation, and translocation within the tissues of higher plants are discussed. Detailed protocols of metal detection with metallochrome indicators dithizone (Cd, Pb), dimethylglyoxime (Ni), sodium rhodizonate (Sr), zincon (Zn), and fluorescent indicator Zinpyr-1 (Zn) by light and fluorescence microscopy are described. Special attention is given to interpretation of the obtained results, advantages and drawbacks of these methods, as well as potential problems associated with histochemical analysis of distribution of heavy metals and strontium.
Bibliography:	http://dx.doi.org/10.1134/S1021443711040133
ISSN:	1021-4437 1608-3407
DOI:	10.1134/S1021443711040133