Magnetically controlled insertion of magnetic nanoparticles into membrane model

• Published in: Biochimica et biophysica acta. Biomembranes Vol. 1866; no. 3; p. 184293
• Main Authors: Moya Betancourt, Sara N., Cámara, Candelaria I., Juarez, Ana V., Riva, Julieta S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-03-2024
• Subjects: Chitosan; Diethyl amino–ethyl dextran; Langmuir monolayer; Magnetic field; Magnetic nanoparticles; Maximum insertion pressure; Chitosan; Langmuir monolayer; Magnetic field; Diethyl amino–ethyl dextran; Magnetic nanoparticles; Maximum insertion pressure
• ISSN: 0005-2736; 1879-2642; 1879-2642
• DOI: 10.1016/j.bbamem.2024.184293

Polysaccharide–coated magnetic nanoparticles (MNPs) have been reported to show potential applications in many biomedical fields. In this report, we have studied the interactions between magnetite (Fe3O4) MNPs functionalized with polysaccharides (diethylamino–ethyl dextran, DEAE–D or chitosan, CHI) with different membranes models by Langmuir isotherms, incorporation experiments, and brewster angle microscopy (BAM). In this report, zwitterionic 1,2–distearoyl–sn–glycerol–3–phosphoethanolamine (DSPE) and anionic 1,2–distearoyl–sn–glycerol–3–phosphate (DSPA) phospholipid, were used to form membrane models. Incorporation experiments (π–t) as well as the compression isotherms demonstrate positive interactions between MNPs and DSPE or DSPA monolayers. The study assessed the impact of varying initial surface pressure on a preformed phospholipid monolayer to determine the maximum insertion pressure (MIP) and synergy. Our findings indicate that the primary driving force of the coated MNPs incorporation into the monolayer predominantly stems from electrostatic interaction. The drop in the subphase pH from 6.0 to 4.0 led to an enhancement of the MIP value for DSPA phospholipid monolayer. On the other hand, for DSPE, the drop in the pH does not affect the MIP values. Besides, the presence of a magnetic field induces an enhancement of the insertion process of the MNPs into DSPA preformed monolayer, demonstrating that a previous interaction between MNPs and phospholipid preformed monolayer needs to take place to enhance the incorporation process. This work opens novel perspectives for the research of the influence of magnetic fields on the incorporation of MNPs into model membranes.
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•There are positive interactions between Fe3O4 with DSPE and DSPA monolayers.•Fe3O4@CHI interacts more strongly with phospholipid molecules than Fe3O4@DEAE–D.•MNPs only decrease the DSPE monolayer stability, mostly for Fe3O4@DEAE–D MNPs.•Both MNPs are incorporated into DSPA and DSPE monolayers.•Magnetic field enhances the insertion of MNPs into the DSPA monolayer.