The effect of gabexate mesilate on the outcome of acute hemorrhagic pancreatitis in pigs

• Published in: Research in experimental medicine Vol. 189; no. 1; pp. 1 - 8
• Main Authors: RISTKARI, S. K. K, RAMO, O. J, KIVINIEMI, H, GROHN, P, MAHLBERG, K
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 1989
• Subjects: Acute Disease; Animals; Biological and medical sciences; Digestive system; Gabexate; Guanidines - pharmacology; Guanidines - therapeutic use; Hemodynamics - drug effects; Hemorrhage - drug therapy; Hemorrhage - mortality; Medical sciences; Pancreatitis - drug therapy; Pancreatitis - mortality; Pharmacology. Drug treatments; Phospholipases A - metabolism; Phospholipases A2; Swine - metabolism; Chemotherapy; Experimental disease; Treatment; Intravenous administration; Enzyme; Animal; Pancreatitis; Enzyme inhibitor; Digestive diseases; Hemodynamics; Hemorrhage; Phospholipase A
• ISSN: 0300-9130; 1433-8580
• DOI: 10.1007/BF01856022

The effects of gabexate mesilate (GM) on hemodynamics and phospholipase A2 activities (PLA2) during acute hemorrhagic pancreatitis (AHP) were studied in 17 piglets which were randomly divided into three groups: The control group (CG) received only the fluid replacement, whereas the pretreatment group (PG) was given an infusion of GM (20 mg/kg/5h), which was started 30 min before and in the treatment group (TG) 30 min after the induction of AHP. AHP was induced by infusing a mixture of trypsin and sodiumtaurocholate (1 ml/kg) into the pancreatic duct, and the animals were followed up for 5h. Two animals of the CG died, but no mortality was observed in the other groups. Histologically, acute hemorrhagic pancreatitis was detected in all animals, but no significant differences were observed between the groups. PLA2 activity in the serum increased rapidly after the induction of AHP in the CG, and it was significantly (P less than 0.05) higher 5h after the induction in the CG than in TG or PG. No significant differences developed between the groups in cardiac indices or hemodynamic pressure parameters during the 5h of surveillance, but the volume of secreted exudate into the peritoneal cavity was significantly (P less than 0.05) smaller in the PG than in the CG. In conclusion, GM treatment and pretreatment reduced mortality and the amount of the secreted ascitic fluid during AHP. Moreover, the activity of circulating PLA2 was inhibited in the groups receiving GM.