Transcriptome Analysis of Multiple Plant Parts in the Woody Oil Tree Camellia drupifera Loureiro

• Published in: Horticulturae Vol. 10; no. 9; p. 914
• Main Authors: Shen, Hongjian, Liao, Boyong, Deng, Jinqing, Liu, Biting, Shen, Yang, Xiong, Wanyu, He, Shan, Zou, Peishan, Chen, Fang, Srihawech, Thitaree, Lee, Shiou Yih, Li, Yongquan
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01-09-2024
• Subjects: Annotations; Branches; Buds; Camellia; Camellia drupifera; Comparative analysis; Flowers; Gene expression; Genes; genetic resources; Industrial products; Leaves; Metabolic pathways; Microorganisms; Pericarp; Plants; Plants (botany); Proteins; Seeds; SMRT sequencing; Subgroups; Theaceae; transcription factor; Transcription factors; Transcriptomes; Trees; China
• ISSN: 2311-7524; 2311-7524
• DOI: 10.3390/horticulturae10090914

Camellia drupifera is mainly used in forestry for its high-value industrial products; however, limited information is available on its transcriptome. This study aimed to construct a full-length transcriptome sequence based on the PacBio sequencing platform for various plant parts of C. drupifera, including flower buds, leaves, leaf buds, branches, the pericarp, and seed kernels. The transcriptomes were annotated with 23,207 genes, with 58 subgroups in the GO classification. The KEGG database revealed 10,407 genes involved in the metabolic pathway analysis, with 68,192 coding sequences, 3352 TF families, 48,541 SSRs, 1421 IncRNAs, and 2625 variable shears predicted. The transcriptomes of different parts were analyzed and compared. The majority of differentially expressed genes (DEGs) were found between the pericarp and seed kernels, followed by leaves and the pericarp with 5662 DEGs, and flower buds and leaf buds with 1616 DEGs. GO and KEGG enrichment analyses showed that KEGG differential genes were significant in microbial metabolism, carbon metabolism, and other functions. The data annotation and analysis of the full-length transcriptome and the comparative analysis between different plant parts provided a theoretical basis for studying gene function, metabolic pathway regulation, and gene expression analysis in KEGG.