Attachment and entry of infectious pancreatic necrosis virus (IPNV) into CHSE-214 cells

• Published in: Archives of virology Vol. 140; no. 10; pp. 1833 - 1840
• Main Authors: KUZNAR, J, SOLER, M, FARIAS, G, ESPINOZA, J. C
• Format: Journal Article
• Language: English
• Published: Wien Springer 01-01-1995; New York, NY
• Subjects: Animals; Biological and medical sciences; Birnaviridae - growth & development; Birnaviridae Infections - physiopathology; Cells, Cultured; Endocytosis; Fishes - microbiology; Fundamental and applied biological sciences. Psychology; Microbiology; Neutralization Tests; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; RNA, Viral - biosynthesis; Time Factors; Virology; Embryo; Virus replication cycle; Birnaviridae; In vitro; Virus; Vertebrata; Cell line; Adsorption; Birnavirus; Internalization; Pisces; Infectivity; Kinetics; Infectious pancreatic necrosis virus
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/BF01384345

Infectious pancreatic necrosis virus (IPNV) attaches to CHSE-214 cells through two types of cell components: specific and non-specific ones. Competition experiments with inactivated IPNV showed that IPNV requires specific components to productively infect cells. Just a low amount of adsorbed IPNV enters the cell. After 20 minutes, part of the adsorbed IPNV was internalized into acid compartments. Also, the viruses adsorbed on the cell surface require similar periods of time to escape from the neutralization of antibodies.