The Thymic Hormone Thymosin-1α Reduces the Pro-Inflammatory Response of Raw 264.7 Cells Induced by Endotoxin

• Published in: Molecular biology (New York) Vol. 57; no. 6; pp. 1004 - 1013
• Main Authors: Novoselova, E. G., Glushkova, O. V., Khrenov, M. O., Lunin, S. M., Sharapov, M. G., Goncharov, R. G., Mubarakshina, E. K., Novoselova, T. V., Parfenyuk, S. B.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-12-2023; Springer Nature B.V
• Subjects: Apoptosis; Biochemistry; Biomedical and Life Sciences; Cell culture; Gene expression; Gram-negative bacteria; Human Genetics; Inflammation; Life Sciences; Lipopolysaccharides; Macrophages; Molecular Mechanisms of Redox Regulation in Inflammation; NF-κB protein; p53 Protein; Thymus; TLR4 protein; Toll-like receptors; cytokines; signaling pathways; inflammation; thymosin-1α; gene expression
• ISSN: 0026-8933; 1608-3245
• DOI: 10.1134/S0026893323060110

—The aim of this work was to study the effects of thymosin-1 alpha (Tα1) on the anti-inflammatory response of RAW 264.7 macrophages cultured in the presence of lipopolysaccharide (LPS) from the walls of gram-negative bacteria. As well, we evaluated production of pro-inflammatory cytokines and the activity of the NF-κB and SAPK/JNK signaling pathways. In addition, the level of expression of a number of genes that regulate cell apoptosis, as well as the activity of receptors involved in the pro-inflammatory response, was determined. First, the addition of Tα1 normalized the level of cytokine production to varying degrees, with a particularly noticeable effect on IL-1β and IL-6. Second, the addition of Tα1 normalized the activity of the NF-κB and SAPK/JNK signaling cascades and the expression of the
Tlr4
gene. Third, Tα1 significantly reduced p53 and the activity of the
P53
gene, which is a marker of cell apoptosis. Fourth, it was shown that the increase in
Ar-1
gene expression under the influence of LPS was significantly reduced using Tα1. Thus, it was found that the presence of Tα1 in the RAW 264.7 cell culture medium significantly reduced the level of the pro-inflammatory response of cells.