Detection of P53 tumor-suppressor-gene protein in bladder tumors and prostate cancer: possible clinical implications

• Published in: World journal of urology Vol. 12; no. 6; pp. 345 - 351
• Main Authors: KUCZYK, M. A, SERTH, J, HERVATIN, C, ARNDT, H, DERENDORF, L, THON, W. F, JONAS, U
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-12-1994; Berlin; New York, NY
• Subjects: Aged; Biological and medical sciences; Carcinoma, Transitional Cell - genetics; Carcinoma, Transitional Cell - pathology; Female; Gene Expression Regulation, Neoplastic; Genes, p53; Humans; Immunohistochemistry; Male; Medical sciences; Neoplasm Staging; Nephrology. Urinary tract diseases; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Prognosis; Prostate - pathology; Prostatic Neoplasms - genetics; Tumor Suppressor Protein p53 - metabolism; Tumors of the urinary system; Urinary Bladder - pathology; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - pathology; Urinary tract. Prostate gland; Histological grading; Human; Immunohistochemistry; Urinary system disease; Prognosis; Prostate disease; Male; Urinary tract disease; Transitional cell carcinoma; Malignant tumor; Gene expression; Pathology; Clinical stage; Urinary bladder; Bladder disease; Male genital diseases; Prostate; Tumor suppressor gene
• ISSN: 0724-4983; 1433-8726
• DOI: 10.1007/BF00184117

For a variety of human malignancies such as breast cancer and cancer of the prostate, p53 oncoprotein overexpression indicating an alteration of the p53 tumor-suppressor gene has been described as a prognostic factor for a poor clinical outcome. To investigate the overexpression of p53 oncoprotein in transitional-cell carcinoma of the bladder, 58 bladder cancer specimens of different clinical stages and histological grades were investigated using an immunohistochemical approach. A correlation between p53 positivity and tumor stage was observed, with an increase from 38.5% of superficial (Ta) tumors to 83.3% of muscle-invasive (T3/T4) tumors staining positively for p53 oncoprotein. Furthermore, an increase from 46.7% of G1 tumors to 75% of G3 tumors was observed. In 22 of 25 (87%) informative patients the results of the immunohistochemical staining could be verified by the determination of p53 mutations as detected by polymerase chain reaction (PCR)-directed analysis of restriction-fragment-length polymorphisms (RFLP). To determine the prognostic value of p53 immunohistochemistry for the clinical course of superficial bladder cancer, the overexpression of p53 oncoprotein was investigated in 41 patients with superficial bladder tumors (T1) undergoing complete transurethral tumor resection. The detection of p53 protein was correlated with further clinically important variables such as sex, age, histological grading, former instillation therapy, and immunohistochemical determination of the proliferation rate by staining for PCNA (proliferating-cell nuclear antigen; monoclonal antibody PC10).