Real-time assays for detection of Phytophthora spp. and identification of an avr3a gene variant

Real-time assays for detection of Phytophthora spp. and identification of an avr3a gene variant

A study was carried out for identification and detection of Phytophthora spp. from soil and plant samples, collected from Solanaceous crops in Egypt and Italy. The samples were screened with specific and universal primers of Phytophthora 18S, ITS1–ITS2 and 28S regions, followed by PCR product sequen...

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Bibliographic Details
Published in:Journal of plant diseases and protection (2006) Vol. 125; no. 3; pp. 331 - 337
Main Authors: Tolba, Shimaa R. T., Sialer, Mariella M. Finetti, Rosso, Laura C., Moustafa, Mahmoud M. A., Ruggeri, Chiara, El-Shawaf, Ibrahim I. S., Ciancio, Aurelio
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Verlag Eugen Ulmer KG 01-06-2018
Springer Berlin Heidelberg
Subjects:
Biomedical and Life Sciences
Life Sciences
Plant Pathology
Plant Physiology
Plant Sciences
SHORT COMMUNICATION
ITS
Molecular beacon
Virulence
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Summary:A study was carried out for identification and detection of Phytophthora spp. from soil and plant samples, collected from Solanaceous crops in Egypt and Italy. The samples were screened with specific and universal primers of Phytophthora 18S, ITS1–ITS2 and 28S regions, followed by PCR product sequencing. The Phytophthora spp. detected were P. infestans (Egypt) and P. parasitica (Italy). A molecular beacon probe was also developed based on the avr3a gene of P. infestans to detect a variant associated with virulence traits. The probe was suitable for avr3a allele identification from P. infestans and also from P. parasitica PCR products.
ISSN:1861-3829
1861-3837
DOI:10.1007/s41348-018-0150-z