IL-1β induces GFAP expression in vitro and in vivo and protects neurons from traumatic injury-associated apoptosis in rat brain striatum via NFκB/Ca2+ –calmodulin/ERK mitogen-activated protein kinase signaling pathway

• Published in: Neuroscience Vol. 252; pp. 367 - 383
• Main Authors: Sticozzi, C, Belmonte, G, Meini, A, Carbotti, P, Grasso, G, Palmi, M
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 12-11-2013; Elsevier
• Subjects: Animals; apoptosis; Apoptosis - physiology; Astrocytes - metabolism; astrogliosis; Biological and medical sciences; Blotting, Western; Calcium - metabolism; Calcium Signaling; Calmodulin - metabolism; Cell Line, Tumor; CNS; Corpus Striatum - injuries; Corpus Striatum - metabolism; Fundamental and applied biological sciences. Psychology; Glial Fibrillary Acidic Protein - biosynthesis; Humans; Immunohistochemistry; interleukin-1beta; Interleukin-1beta - metabolism; Male; MAP Kinase Signaling System - physiology; Mitogen-Activated Protein Kinases - metabolism; Nerve Degeneration - metabolism; Neurology; Neurons - metabolism; NF-kappa B - metabolism; Rats; Rats, Sprague-Dawley; signal transduction; Signal Transduction - physiology; Vertebrates: nervous system and sense organs; 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid; NF-kB; PFA; phosphate-buffered saline; calmodulin; 2-APB; CaM; neuronal nuclei; BSA; ryanodine; iNOS; paraformaldehyde; inositol-(1,4,5)-trisphosphate; 1400W; extracellular regulated mitogen-activated protein kinases; U0126; PBS; nitric oxide; IL; SDS; signal transduction; 1,4-diazabicyclo octane in glycerine; [Ca 2+] i; DAPI; 4′,6-diamidino-2-phenylindole; EGTA; ERK1/2; NeuN; FCS; ethylene glycol tetraacetic acid; glial fibrillary acidic protein; bovine serum albumin; W5; nuclear factor-kB; W7; NO; RT; room temperature; tetramethylrhodamine-isothiocyanate conjugated; intracellular Ca 2+ concentration; RY; CNS; apoptosis; astrogliosis; CAPE; DABCO; interleukin-1beta; inducible nitric oxide synthase; FITC; chondroitin sulfate proteoglycan; 1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthiol]-butadiene; fluorescein isothiocyanate conjugated; IP 3; N-(6-aminohexyl)-5-chloro-1-naphthalensulfonamide hydrochloride; sodium dodecyl sulfate; fetal calf serum; N-(6-aminohexyl)-1-naphthalensulfonamide hydrochloride; N-[(3-(aminomethyl)-phenyl]methyl]-ethanimidamide dihydrochloride; 2-aminoethoxydiphenylborate; GFAP; interleukin-1 beta; HEPES; CSPG; caffeic-acid phenethyl-ester; TRITC; [Ca2+]i; IP3; Calcium; Rat; Neuroglia; Encephalon; Transcription factor NFκB; Calmodulin; Glial fibrillary acidic protein; Cytokine; Rodentia; Interleukin 1β; Basal ganglion; Corpus striatum; In vitro; Trauma; Vertebrata; Mammalia; Neuron; Cell death; Animal; Lesion; Apoptosis; IP; [Ca(2+)](i); intracellular Ca(2+) concentration
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2013.07.061

Highlights • Mechanisms of IL-1β regulation and functions of astrocyte activation are proposed. • (NF-kB)/Ca2+ /calmodulin/ERK1/2 signaling is a novel key regulator of astrogliosis. • In contrast to high, moderate astrocyte activation protects neurons from apoptosis. • The identified signaling may represent a potential target in neurodegeneration.