A combination of resveratrol and 3,3'-diindolylmethane, a potent radioprotector

• Published in: International journal of radiation biology Vol. 94; no. 6; pp. 558 - 568
• Main Authors: Thekkekkara, Dithu, Basavan, Duraiswamy, Chandna, Sudhir, Nanjan, Moola Joghee
• Format: Journal Article
• Language: English
• Published: England 01-06-2018
• Subjects: Animals; Antioxidants - metabolism; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Bone Marrow Cells - radiation effects; Chromosomes - drug effects; Chromosomes - radiation effects; Drug Interactions; Hematopoiesis - drug effects; Hematopoiesis - radiation effects; Indoles - pharmacology; Male; Mice; Radiation-Protective Agents - pharmacology; Resveratrol - pharmacology; chromosomal aberrations; Ionizing radiation; radioprotection; 3,3′-diindolylmethane; micronuclei; resveratrol
• ISSN: 0955-3002; 1362-3095
• DOI: 10.1080/09553002.2018.1467063

Exposure to ionizing radiation causes damage to the genomic integrity and stability of the cell. Though a large number of molecules have been studied for their radioprotective capability, no single agent is available today that meets all the requirements of a good radiprotector. In this study, we have investigated a combination of Resveratrol (RSV) and 3,3'-Diindolyl methane (DIM) for its efficacy for radioprotection. It is our hypothesis that this combination that possesses less toxicity than synthetic compounds, free radical scavenging potential, and the capacity to interfere with the several of the signaling cascades that trigger damage to cell by ionizing radiation may possess good radioprotective capability.
Mice were pre-treated with a combination of RSV and DIM and the 30-day mortality assay, endogenous antioxidant levels in intestinal mucosa, metaphase chromosomal aberrations, and micronuclei formation were assessed after exposed to ionizing radiation.
The dose modifying factor (DRF) obtained for RSV, DIM, and the combination is 1.15, 1.17, and 1.3, respectively. Pre-treatment of mice with the combination results in significant (***p = .001) protection of the endogenous antioxidant levels, chromosomal aberrations, micronuclei formation, after exposure to ionizing radiation.
Our findings suggest that pre-treatment with the combination of RSV and DIM protects effectively from the ionizing radiation-induced damage at the molecular, cellular, and tissue levels by counteracting both the direct and indirect effects.